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Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp.

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Abstract

Festuca L. has an important diversification centre in the Iberian Peninsula. We used chromosome counting, fluorescence (FISH) and genomic in situ hybridization (GISH), and DNA flow cytometry (FCM) to clarify the taxonomic position of several taxa, to search for phylogenetic relationships and to assess the extent and pattern of genome variation in fescues. The chromosome number of Festuca duriotagana var. barbata is determined for the first time and new ploidy level estimations are given for F. rothmaleri and F. summilusitana. In the latter species, besides the reported decaploid level, dodecaploidy was found in some populations, which points to the existence of an unrecognized taxon. Moreover, these differences were confirmed by FCM and a high positive correlation was found with the type of substrate where F. summilusitana was growing. For each section, a decrease of genome size with increase of polyploidy was observed. In general, in situ hybridization techniques failed to reveal phylogenetic relationships among the selected species. In FISH, a variation in the number of rDNA sites was observed in some species. GISH results indicate that F. henriquesii is not a progenitor of the studied polyploid species.

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References

  • Al-Bermani AK, Catalan P and Stace CA (1992). A new circumscription of Festuca trichophylla (Gaudin) K. Richter (Gramineae). An Jard Bot Anales Jard. Bot. Madrid 50: 209–220

    Google Scholar 

  • Anonymous (2006) Plano Sectorial da Rede Natura 2000. ICN. Website: http://www.icn.pt/psrn2000/caracterizacao_valores_naturais/flora/Festuca%20duriotagana.pdf

  • Arumuganathan K, Tallury SP, Fraser ML, Bruneau AH and Qu R (1999). Nuclear DNA content of thirteen turfgrass species by flow cytometry. Crop Sci 39: 1518–1521

    Google Scholar 

  • Bennett MD and Leitch IJ (2005). Nuclear DNA amounts in Angiosperms: progress, problems and prospects. Ann Bot 95: 45–90

    Article  PubMed  CAS  Google Scholar 

  • Bennett MD, Smith JB and Smith RIL (1982). DNA Amounts of angiosperms from the Antarctic and South Georgia. Environ Exp Bot 22: 307–318

    Article  Google Scholar 

  • Cebolla Lozano C and Rivas Ponce MA (2003). Catálogo del género Festuca L. (Poaceae) en la Península Ibérica. Candollea 58: 189–213

    Google Scholar 

  • Ceccarelli M, Falistocco E and Cionini PG (1992). Variation of genome size and organization within hexaploid Festuca arundinacea. Theor Appl Genet 83: 273–278

    Article  Google Scholar 

  • de la Fuente V and Ortúñez E (2000). Nueva especie de Festuca L. sección Festuca (Poaceae) en la Península Ibérica. Lazaroa 21: 3–6

    Google Scholar 

  • de la Fuente V and Ortúñez E (2001). Festuca sect. Eskia (Poaceae) in the Iberian Peninsula. Folia Geobot 36: 385–421

    Article  Google Scholar 

  • de la Fuente V, Ortúñez E and Ferrero LM (1997). Contribución al conocimiento del género Festuca L. (Poaceae) en el País Basco y Sistema Ibérico septentrional (Península Ibérica). Itin Geobot 10: 317–351

    Google Scholar 

  • de la Fuente V, Ferrero LM and Ortúñez E (2001). Chromosome counts in the genus Festuca L. section Festuca (Poaceae) in the Iberian Peninsula. Bot J Linn Soc 137: 385–398

    Article  Google Scholar 

  • Devesa JA, Ruiz T, Tormo R, Muñoz A, Viera MC, Carrasco JP, Ortega A and Pastor J (1990). Contribución al conocimiento cariológico de las Poaceae en Extremadura (España) II. Bol Soc Brot, Sér 2 63: 153–205

    Google Scholar 

  • Doležel J (1997). Applications of flow cytometry for the study of plant genomes. J Appl Genet 38: 285–302

    Google Scholar 

  • Doležel J, Bartoš J, Voglmayr H and Greilhuber J (2003). Nuclear DNA content and genome size of trout and human. Cytometry Part A 51A: 127–128

    Article  Google Scholar 

  • Doležel J, Greilhuber J, Lucretti S, Meister A, Lysák M, Nardi L and Obermayer R (1998). Plant genome size estimation by flow cytometry: inter-laboratory comparison. Ann Bot 82: 17–26

    Article  Google Scholar 

  • Dubcovsky J and Martínez A (1992). Distribución geográfica de los niveles de ploidía en Festuca. Parodiana 7: 91–99

    Google Scholar 

  • Ferrero LM and de la Fuente V (1996). Aportaciones al estudio cariológico de algunas especies del género Festuca L. endémicas del Mediterráneo Occidental. Bol Soc Brot 67: 303–308

    Google Scholar 

  • Foggi B, Rossi G, Signorini MA, Pignotti L, Parolo G (2005) Il genere Festuca L. (Poaceae) in Italia. Website: http://www.unipv.it/labecove/Festuca/Home.htm

  • Franco JA and Rocha Afonso ML (1998). Nova flora de Portugal (Continente e Açores) - Gramineae, Vol III. Escolar Editora, Lisboa, Portugal

    Google Scholar 

  • Fukui K, Kamisugi Y and Sakai F (1994). Physical mapping of 5S rDNA loci by direct-cloned biotinylated probes in barley chromosomes. Genome 37: 105–111

    PubMed  CAS  Google Scholar 

  • Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP and Firoozabady E (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051

    Article  PubMed  CAS  Google Scholar 

  • Galland N (1988). Recherche sur l'origine de la flore orophile du Maroc: étude caryologique et cytogéographique. Travaux de l'Institut Scientifique, Université Mohammed V. Sér Bot 35: 1–168

    Google Scholar 

  • Gerlach WL and Bedbrook JR (1979). Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl Acids Res 7: 1869–1885

    Article  PubMed  CAS  Google Scholar 

  • Greilhuber J, Doležel J, Lysák MA and Bennett MD (2005). The origin, evolution and proposed stabilization of the terms `genome size' and `C-value' to describe nuclear DNA contents. Ann Bot 95: 255–260

    Article  PubMed  CAS  Google Scholar 

  • Grime JP and Mowforth MA (1982). Variation in genome size - an ecological interpretation. Nature 299: 151–153

    Article  Google Scholar 

  • Harper JA, Thomas ID, Lovatt JA and Thomas HM (2004). Physical mapping of rDNA sites in possible diploid progenitors of polyploid Festuca species. Pl Syst Evol 245: 163–168

    Article  CAS  Google Scholar 

  • Huff DR and Palazzo AJ (1998). Fine fescue species determination by laser flow cytometry. Crop Sci 38: 445–450

    Article  Google Scholar 

  • Humphreys MW, Thomas HM, Morgan WG, Meredith MR, Harper JA, Thomas H, Zwierzykowski Z and Ghesquière M (1995). Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization. Heredity 75: 171–174

    Google Scholar 

  • Kerguélen M (1975). Les Gramineae (Poaceae) de la flore Francaise. Essai de mise au point taxonomique et nomenclaturale. Lejeunia, Nouv Sér 75: 1–343

    Google Scholar 

  • Konarska B (1974). Karyological studies on Festuca rubra L. s.l. from Poland. Acta Biol Cracov Ser Bot 17: 175–118

    Google Scholar 

  • Kopecký D, Lukaszewski AJ and Gibeault V (2005a). Reduction of ploidy level by androgenesis in intergeneric Lolium-Festuca hybrids for turf grass breeding. Crop Sci 45: 274–281

    Google Scholar 

  • Kopecký D, Lukaszewski AJ and Doležel J. (2005b). Genomic constitution of Festulolium cultivars released in the Czech Republic. Pl Breed 124: 454–458

    Article  Google Scholar 

  • Kopecký D, Loureiro J, Zwierzykowski Z, Ghesquière M and Doležel J (2006). Genome constitution and evolution in Lolium x Festuca hybrid cultivars (Festulolium). Theor Appl Genet 113: 731–742

    Article  PubMed  CAS  Google Scholar 

  • Kosmala A, Zwierzykowski Z, Gasior D, Rapacz M, Zwierzykowska E and Humphreys MW (2006). GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum. Heredity 96: 243–251

    Article  PubMed  CAS  Google Scholar 

  • Leitch IJ and Bennett MD (2004). Genome downsizing in polyploid plants. Biol J Linn Soc 82: 651–663

    Article  Google Scholar 

  • Malik CP and Thomas PT (1966). Karyotypic studies in some Lolium and Festuca species. Caryologia 19: 167–195

    Google Scholar 

  • Masoudi-Nejad A, Nasuda S, McIntosh RA and Endo TR (2002). Transfer of rye chromosome segments to wheat by a gametocidal system. Chromosome Res 10: 349–357

    Article  PubMed  CAS  Google Scholar 

  • Ortúñez E, de la Fuente V (1995) Reports (394–400) In: Kamari G, Felber F, Garbari F (eds) Mediterranean chromosome number reports. 5. Flora Mediterranea 5: 261–265

  • Ortúñez E and de La Fuente V (2004). Chromosome counts in the genus Festuca section Eskia (Poaceae) in the Iberian Peninsula. Bot J Linn Soc 146: 331–337

    Article  Google Scholar 

  • Pfosser M, Amon A, Lelley T and Heberle-Bors E (1995). Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21: 387–393

    Article  PubMed  CAS  Google Scholar 

  • Portal R (1999) Festuca de France. Published by the Author, Le Puy-en-Velay

  • Queirós M (1973) Contribuição para o conhecimento citotaxonómico das Spermatophyta de Portugal I. Gramineae, supl. 2. Bol Soc Brot, Sér 2 47: 77–103

  • Raina SN and Rani V (2001). GISH technology in plant genome research. Methods in Cell Biol 23: 83–104

    Article  CAS  Google Scholar 

  • Schifino MT and Winge H (1983). Systematics and evolution of the Briza complex (Gramineae). 2. Karyotypes and nuclear DNA content of species of the Briza complex and some other genera of Poeae (Gramineae). Revista Brasileira de Genet. Poeae 6: 245–259

    Google Scholar 

  • Schubert I and Wobus U (1985). In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92: 143–148

    Article  Google Scholar 

  • Schwarzacher T and Heslop-Harrison P (2000). Practical in Situ Hybridization. BIOS Scientific Publishers, Oxford, United Kingdom

    Google Scholar 

  • Schwarzacher T, Leitch AR, Bennett MD and Heslop-Harrison JS (1989). In situ localization of parental genomes in a wide hybrid. Ann Bot 64: 315–324

    Google Scholar 

  • Seal AG (1983). DNA Variation in Festuca. Heredity 50: 225–236

    CAS  Google Scholar 

  • Shaked H, Kashkush K, Ozkan H, Feldman M and Levy AA (2001). Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Pl Cell 13: 1749–1759

    CAS  Google Scholar 

  • Šmarda P and Bureš P (2006). Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels. Ann Bot 98: 665–678

    Article  PubMed  Google Scholar 

  • Takahashi C, Marshall JA, Bennett MD and Leitch IJ (1999). Genomic relationships between maize and its wild relatives. Genome 42: 1201–1207

    Article  PubMed  CAS  Google Scholar 

  • Thomas HM, Harper JA, Meredith MR, Morgan WG and King IP (1997). Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization. Genome 40: 406–410

    Article  CAS  PubMed  Google Scholar 

  • Thomas HM, Harper JA, Meredith MR, Morgan WG, Thomas ID, Timms E and King IP (1996). Comparison of ribosomal DNA sites in Lolium species by fluorescence in situ hybridization. Chromosome Res 4: 486–490

    Article  PubMed  CAS  Google Scholar 

  • Xu W and Sleper DA (1994). Phylogeny of tall fescue and related species using RFLPs. Theor Appl Genet 88: 685–690

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. CESAM & Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    J. Loureiro, S. Castro, C. Santos & P. Silveira

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

    D. Kopecký

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  1. J. Loureiro
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  2. D. Kopecký
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  3. S. Castro
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  4. C. Santos
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  5. P. Silveira
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Correspondence to J. Loureiro.

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Loureiro, J., Kopecký, D., Castro, S. et al. Flow cytometric and cytogenetic analyses of Iberian Peninsula Festuca spp.. Plant Syst. Evol. 269, 89–105 (2007). https://doi.org/10.1007/s00606-007-0564-8

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  • DOI: https://doi.org/10.1007/s00606-007-0564-8

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