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Multifunctional lanthanide metal-organic framework based ratiometric fluorescence visual detection platform for alkaline phosphatase activity

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Abstract

A turn-on/off ratiometric fluorescence detection platform based on multifunctional lanthanide metal-organic framework (Ln-MOF) and an enzymatic cascade reaction is proposed for alkaline phosphatase (ALP) activity assay. L-phosphotyrosine is hydrolyzed to levodopa (L-dopa) by two steps of enzymatic reaction. L-dopa further reacts with naphthoresorcinol to produce carboxyazamonardine with strong emission at 490 nm. In this process, multifunctional Ln-MOF (Cu@Eu-BTC, BTC is the 1,3,5-benzenetricarboxylic acid) acts not only as a nanozyme to catalyze the fluorogenic reaction between L-dopa and naphthoresorcinol but also as a fluorescence internal standard. The emission of Cu@Eu-BTC at 620 nm is quenched by phosphate anions, and the dual-response ratiometric fluorescence (F490/F620) can be achieved. A good linear relationship was obtained between Δ(F490/F620) and ALP activity in the range 0.3–24 U L−1 with the detection limit of 0.02 U L−1. In addition, a portable assay tube was designed for visual and point-of-care testing of ALP activity by color variation (ratiometric chromaticity). Both the ratiometric fluorescence detection and the visual detection methods were successfully applied to monitor ALP activity in human serum samples with recovery between 95.5%–109.0% and 94.0%–110.1%, and relative standard deviation less than 8.1% and 9.5%, respectively. As far as we know, this is the first report of ALP activity assay assisted by multifunctional Ln-MOF.

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Acknowledgements

We thank the School of Chemistry and Molecular Science, Wuhan University, for their supply of the Materials Studio software.

Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 82073811 and 81673394) and the Fundamental Research Funds for the Central Universities (Grant No. 2042020kf1010).

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  1. Li Xiong and Long Yu are the co-first authors.

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  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China

    Li Xiong, Long Yu, Shuo Li, Lixiang Feng & Yuxiu Xiao

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Xiong, L., Yu, L., Li, S. et al. Multifunctional lanthanide metal-organic framework based ratiometric fluorescence visual detection platform for alkaline phosphatase activity. Microchim Acta 188, 236 (2021). https://doi.org/10.1007/s00604-021-04880-4

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