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Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine

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Abstract

It is demonstrated that vanadium tetrasulfide (VS4) exhibits peroxidase (POx)-like activity which follows Michaelis-Menten kinetics when using H2O2 as a co-substrate. Electron spin resonance spectroscopy was use to analyze the catalytic mechanism. It suggests that the enzyme mimicking activity is caused by decomposing H2O2 into hydroxyl radicals. The method was used to quantify H2O2 by using 3,3′,5,5′-tetramethylbenzidine as the substrate which results in the formation of a blue coloration (with an absorption peak at 652 nm). H2O2 can be detected in the 50 to 300 μM concentration range, and the detection limit is 5.0 μM. The assay for L-cysteine (L-cys) is based on the capability of oxTMB to oxidize L-cys to form L-cystine. The colorimetric L-cys assay has a linear response in the 5 to 100 μM concentration range and a 2.5 μM detection limit.

Schematic representation of the enzyme mimicking activity of vanadium tetrasulfide (VS4) submicrospheres originated from the decomposition of hydrogen peroxide (H2O2) to generate reactive hydroxyl radical (·OH) and the colorimetric detection of L-cysteine.

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Acknowledgments

This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Grant No. XDA23050104), Natured Science Foundation of China (Grant No. 41776090), Key Research and Development Program of Shandong Province (Grant Nos. 2018GHY115038 and 2018GGX104021) and AoShan Talent Program Supported by Qingdao National Laboratory for Marine Science and Technology.

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Authors and Affiliations

  1. CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China

    Chao Chen, Yi Wang & Dun Zhang

  2. University of Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing, 100039, China

    Chao Chen

  3. Open Studio for Marine Corrosion and Protection, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, 266237, China

    Chao Chen, Yi Wang & Dun Zhang

  4. Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China

    Chao Chen, Yi Wang & Dun Zhang

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  1. Chao Chen
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Correspondence to Yi Wang or Dun Zhang.

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Chen, C., Wang, Y. & Zhang, D. Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine. Microchim Acta 186, 784 (2019). https://doi.org/10.1007/s00604-019-3942-3

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