Skip to main content
SpringerLink
Log in

Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A visualization strategy is described for the detection of clenbuterol (CLB). It is using of antibody against dsDNA and G-quadruplex/hemin labeled on a metal organic framework of type MIL-101(Fe) (G-quadruplex/hemin-anti-DNA/MIL-101) acting as a peroxidase mimetic, and magnetic beads modified with aptamer and complementary DNA (MB/Apt-cDNA) as capture probes. The detection reagent was prepared via the reactions between the double stranded DNA (Apt-cDNA) in capture probes and anti-DNA in peroxidase mimetic. In the presence of CLB, the aptamer on the magnetic beads preferentially binds CLB, and the peroxidase mimetic is released to the supernatant after magnetic separation. The released peroxidase mimetic can catalyze the TMB/H2O2 chromogenic system under mild conditions. This leads to the development of a blue-green coloration whose absorbance is measured at 650 nm. The detection limit is as low as 34 fM of CLB. The method was applied to the determination of CLB in pork samples and gave results that were consistent with data obtained with an ELISA kit.

A visualization strategy is described for the detection of clenbuterol. The selectivity of detection system for clenbuterol is excellent compared with other interferents. The method was applied to the determination of CLB in pork samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Zhang Z, Duan F, He L, Peng D, Yan F, Wang M, Zong W, Jia C (2016) Electrochemical clenbuterol immunosensor based on a gold electrode modified with zinc sulfide quantum dots and polyaniline. Microchim Acta 183(3):1089–1097

    Article  CAS  Google Scholar 

  2. Shellaiah M, Simon T, Venkatesan P, Sun KW, Ko FH, Wu SP (2018) Nanodiamonds conjugated to gold nanoparticles for colorimetric detection of clenbuterol and chromium (III) in urine. Microchim Acta 185(1):74

    Article  Google Scholar 

  3. Ji R, Chen S, Xu W, Qin Z, Qiu JF, Li CR (2018) A voltammetric immunosensor for clenbuterol based on the use of a MoS 2-AuPt nanocomposite. Microchim Acta 185(4):209

    Article  Google Scholar 

  4. Nieminen MS, Rämö MP, Viitasalo M, Heikkilä P, Karjalainen J, Mäntysaari M, Heikkila J (1996) Serious cardiovascular side effects of large doses of anabolic steroids in weight lifters. Eur Heart J 17(10):1576–1583

    Article  CAS  Google Scholar 

  5. Cubria JC, Reguera R, Balana-Fouce R, Ordonez C, Ordonez D (1998) Biochemical pharmacology: polyamine-mediated heart hypertrophy induced by Clenbuterol in the mouse. J Pharm Pharmacol 50(1):91–96

    Article  CAS  Google Scholar 

  6. Baronti A, Grieco A, Vibelli C (1980) Oral NAB 365 (clenbuterol) and terbutaline in chronic obstructive lung disease: a double-blind, two-week study. Int J Clin Pharmacol Ther Toxicol 18(1):21–25

    CAS  PubMed  Google Scholar 

  7. Liu B, Yan H, Qiao F, Geng Y (2011) Determination of clenbuterol in porcine tissues using solid-phase extraction combined with ultrasound-assisted dispersive liquid-liquid microextraction and HPLC-UV detection. J Chromatogr B 879(1):90–94

    Article  CAS  Google Scholar 

  8. Ye D, Wu S, Xu J, Jiang R, Zhu F, Ouyang G (2015) Rapid determination of clenbuterol in pork by direct immersion solid-phase microextraction coupled with gas chromatography-mass spectrometry. J Chromatogr Sci 54(2):112–118

    PubMed  Google Scholar 

  9. Rafique R, Baek SH, Nguyen TP, Park KY, Kim EB, Kim JG, Park JP, Suresh Kailasa SK, Kim HJ, Chung C, Shim TS, Park TJ (2018) Gold-copper nanoshell dot-blot immunoassay for naked-eye sensitive detection of tuberculosis specific CFP-10 antigen. Biosens Bioelectron 121:111–117

    Article  Google Scholar 

  10. Du W, Lei C, Zhang S, Bai G, Zhou H, Sun M, Chang C (2014) Determination of clenbuterol from pork samples using surface molecularly imprinted polymers as the selective sorbents for microextraction in packed syringe. J Pharm Biomed Anal 91:160–168

    Article  CAS  Google Scholar 

  11. Deng SL, Shan S, Xu CL, Liu DF, Xiong YH, Wei H, Lai WH (2014) Sample preincubation strategy for sensitive and quantitative detection of clenbuterol in swine urine using a fluorescent microsphere–based immunochromatographic assay. J Food Prot 77(11):1998–2003

    Article  Google Scholar 

  12. Yan F, Zhang Y, Zhang S, Zhao J, Liu S, He L, Feng X, Zhang H, Zhang Z (2015) Carboxyl-modified graphene for use in an immunoassay for the illegal feed additive clenbuterol using surface plasmon resonance and electrochemical impedance spectroscopy. Microchim Acta 182(3-4):855–862

    Article  CAS  Google Scholar 

  13. Baek SH, Kim MW, Park CY, Choi CS, Kailasa SK, Park JP, Park TJ (2019) Development of a rapid and sensitive electrochemical biosensor for detection of human norovirus via novel specific binding peptides. Biosens Bioelectron 123:223–229

    Article  CAS  Google Scholar 

  14. Yan P, Tang Q, Deng A, Li J (2014) Ultrasensitive detection of clenbuterol by quantum dots based electrochemiluminescent immunosensor using gold nanoparticles as substrate and electron transport accelerator. Sensors Actuators B Chem 191:508–515

    Article  CAS  Google Scholar 

  15. Simon T, Shellaiah M, Steffi P, Sun KW, Ko FH (2018) Development of extremely stable dual functionalized gold nanoparticles for effective colorimetric detection of clenbuterol and ractopamine in human urine samples. Anal Chim Acta 1023:96–104

    Article  CAS  Google Scholar 

  16. Kang J, Zhang Y, Li X, Miao L, Wu A (2015) A rapid colorimetric sensor of clenbuterol based on cysteamine-modified gold nanoparticles. ACS Appl Mater Interfaces 8(1):1–5

    Article  Google Scholar 

  17. Bui QA, Vu THH, Ngo VKT, Kennedy IR, Lee NA, Allan R (2016) Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design. Anal Bioanal Chem 408:6045–6052

    Article  CAS  Google Scholar 

  18. Hu X, Zhang H, Chen S, Yuan R, You J (2018) A signal-on electrochemiluminescence sensor for clenbuterol detection based on zinc-based metal-organic framework–reduced graphene oxide–CdTe quantum dot hybrids. Anal Bioanal Chem 410(30):7881–7890

    Article  CAS  Google Scholar 

  19. Fei ZX, Zhang M, Xie SM, Yuan LM (2014) Capillary electrochromatographic fast enantioseparation based on a chiral metal–organic framework. Electrophoresis 35(24):3541–3548

    Article  CAS  Google Scholar 

  20. James SL (2003) Metal-organic frameworks. Chem Soc Rev 32(5):276–288

    Article  CAS  Google Scholar 

  21. Fan C, Lv X, Liu F, Feng L, Liu M, Cai Y, Liu H, Wang J, Yang J, Wang H (2018) Silver nanoclusters encapsulated into metal-organic frameworks with enhanced fluorescence and specific ion accumulation toward the microdot Array-based Fluorimetric analysis of copper in blood. ACS Sensors 3(2):441–450

    Article  CAS  Google Scholar 

  22. Paschke B, Wixforth A, Denysenko D, Volkmer D (2017) Fast surface acoustic wave-based sensors to investigate the kinetics of gas uptake in ultra-microporous frameworks. ACS Sensors 2(6):740–747

    Article  CAS  Google Scholar 

  23. Pelossof G, Tel-Vered R, Willner I (2012) Amplified surface plasmon resonance and electrochemical detection of Pb2+ ions using the Pb2+-dependent DNAzyme and hemin/G-quadruplex as a label. Anal Chem 84(8):3703–3709

    Article  CAS  Google Scholar 

  24. Kong DM, Wu J, Wang N, Yang W, Shen HX (2009) Peroxidase activity–structure relationship of the intermolecular four-stranded G-quadruplex–hemin complexes and their application in hg 2+ ion detection. Talanta 80(2):459–465

    Article  CAS  Google Scholar 

  25. Emlen W, Ansari R, Burdick G (1984) DNA-anti-DNA immune complexes. Antibody protection of a discrete DNA fragment from DNase digestion in vitro. J Clin Investig 74(1):185–190

    Article  CAS  Google Scholar 

  26. Toh SY, Citartan M, Gopinath SC, Tang TH (2015) Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay. Biosens Bioelectron 64:392–403

    Article  CAS  Google Scholar 

  27. Duan N, Gong W, Wu S, Wang Z (2017) Selection and application of ssDNA aptamers against Clenbuterol hydrochloride based on ssDNA library immobilized SELEX. J Agric Food Chem 65(8):1771–1777

    Article  CAS  Google Scholar 

  28. Zhou XH, Kong DM, Shen HX (2009) Ag+ and cysteine quantitation based on G-quadruplex− hemin DNAzymes disruption by ag+. Anal Chem 82(3):789–793

    Article  Google Scholar 

  29. Liu Y, Gao P, Huang C, Li Y (2015) Shape-and size-dependent catalysis activities of iron-terephthalic acid metal-organic frameworks. SCIENCE CHINA Chem 58(10):1553–1560

    Article  CAS  Google Scholar 

  30. Yang Y, Cheng J, Wang B, Guo Y, Dong X, Zhao J (2019) An amino-modified metal-organic framework (type UiO-66-NH2) loaded with cadmium (II) and lead (II) ions for simultaneous electrochemical immunosensing of triazophos and thiacloprid. Microchim Acta 186(2):101

    Article  Google Scholar 

  31. Wang R, Zhou X, Zhu X, Yang C, Liu L, Shi H (2017) Isoelectric bovine serum albumin: robust blocking agent for enhanced performance in optical-fiber based DNA sensing. ACS Sensors 2(2):257–262

    Article  CAS  Google Scholar 

  32. Jin X, Fang G, Pan M, Yang Y, Bai X, Wang S (2018) A molecularly imprinted electrochemiluminescence sensor based on upconversion nanoparticles enhanced by electrodeposited rGO for selective and ultrasensitive detection of clenbuterol. Biosens Bioelectron 102:357–364

    Article  CAS  Google Scholar 

  33. Zhang X, Zhao H, Xue Y, Wu Z, Zhang Y, He Y, Li X, Yuan Z (2012) Colorimetric sensing of clenbuterol using gold nanoparticles in the presence of melamine. Biosens Bioelectron 34(1):112–117

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We acknowledge financial support from State Administration of Traditional Chinese Medicine of Guizhou Province (Contract No. QZYY-2018-094), the Natural Science Foundation of Guizhou, China (No.2019-1329) and the Doctoral Program of Zunyi Medical University (Contract No.F-869).

Author information

Authors and Affiliations

  1. School of Pharmacy, Zunyi Medical University, Guizhou, 563000, China

    Yuandong Zhang

  2. School of Chemistry and Chemical Engineering, Zunyi Normal College, Guizhou, 563006, China

    Hong-Xia Ren

  3. Department of Chemical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, Taiwan, 30013

    Yang-Bao Miao

Authors
  1. Yuandong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong-Xia Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yang-Bao Miao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hong-Xia Ren.

Ethics declarations

The author(s) declare that they have no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOC 401 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Ren, HX. & Miao, YB. Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic. Microchim Acta 186, 515 (2019). https://doi.org/10.1007/s00604-019-3604-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-019-3604-5

Keywords

Search

Navigation