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A fluorometric assay for oxytetracycline based on the use of its europium(III) complex and aptamer-modified silver nanoparticles

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Abstract

An ultrasensitive assay is described for the determination of oxytetracycline (OTC) at nanomolar levels. The method is using silver nanoparticles (AgNPs) that were first modified with OTC-binding aptamer and then exposed to the OTC-Eu(III) complex. The pink fluorescence of the OTC-Eu(III) complex on the AgNPs is almost completely quenched. On addition of OTC, it will compete with the OTC-Eu(III) complex for binding to the aptamer on the AgNPs. The OTC-Eu(III) complex is released and becomes strongly fluorescent, with excitation/emission peaks at 385/620 nm. The resulting assay was validated in terms of linearity and linear range, sensitivity, selectivity, detection limit and accuracy. Under optimum conditions, response is linear in the 10 to 500 nM OTC concentration range, and the limit of detection is 1.9 nM. The method was applied to the determination of OTC in spiked milk and tablets samples, and it gave satisfactory results.

Schematic presentation of the assay. In the presence of oxytetracycline (OTC), it will compete with the OTC-europium(III) complex for binding to the silver nanoparticles (AgNPs)-aptamer conjugate. The OTC-Eu(III) complex is released and strong pink fluorescence is observed.

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Acknowledgments

Financial support of this study was provided by the Mashhad University of Medical Sciences (grant number: 951099) and the Ferdowsi University of Mashhad (grant number: 3/42232).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, 91779-48974, Iran

    Masoomeh Esmaelpourfarkhani & Mahmoud Chamsaz

  2. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran

    Khalil Abnous

  3. Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran

    Khalil Abnous

  4. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran

    Seyed Mohammad Taghdisi

  5. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 91778-99191, Iran

    Seyed Mohammad Taghdisi

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  1. Masoomeh Esmaelpourfarkhani
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  2. Khalil Abnous
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  3. Seyed Mohammad Taghdisi
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  4. Mahmoud Chamsaz
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Correspondence to Khalil Abnous, Seyed Mohammad Taghdisi or Mahmoud Chamsaz.

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Esmaelpourfarkhani, M., Abnous, K., Taghdisi, S.M. et al. A fluorometric assay for oxytetracycline based on the use of its europium(III) complex and aptamer-modified silver nanoparticles. Microchim Acta 186, 290 (2019). https://doi.org/10.1007/s00604-019-3389-6

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  • DOI: https://doi.org/10.1007/s00604-019-3389-6

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