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Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin

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Abstract

An aptamer based impedimetric assay for the mycotoxin patulin (PAT) is described. A glassy carbon electrode (GCE) was modified with black phosphorus nanosheets (BP NSs) and modified with PAT aptamer by electrostatic attraction. Detection is based on the variations of electron transfer resistance at the modified electrode surface. This assay can detect PAT over a linear range that extends from 1.0 nM to 1.0 μM with a 0.3 nM detection limit. To improve the performance of the sensor, the BP NS-GCE was further modified with gold nanoparticles and then with thiolated PAT aptamer. This modified electrode, operated at an applied potential of 0.18 V (vs. Ag/AgCl), has a wider linear range (0.1 nM to 10.0 μM) and a lower detection limits (0.03 nM). Both assays were successfully applied to the analysis of (spiked) genuine food samples.

Black phosphorus nanosheets (BP NSs) were used to fabricate an aptamer based assay for patulin. To further improve the performance of the electrode, gold nanoparticles (AuNP) were placed on the surface of black phosphorus nanosheets (AuNP-BP NSs) by electrostatic attraction for patulin aptasensing.

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Acknowledgments

The authors gratefully acknowledge the financial support of this project by the National Science Foundation of China (21575113), the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1811), the Natural Science Foundation of Shaanxi Province in China (2017JM2036).

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Authors and Affiliations

  1. College of Chemistry & Materials Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, 710069, Shaanxi, China

    Jinqiong Xu, Xiujuan Qiao & Jianbin Zheng

  2. College of Food Science and Engineering, Northwest University, Xi’an, 710069, Shaanxi, China

    Yuan Wang, Qinglin Sheng & Tianli Yue

  3. State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 210023, Jiangsu, China

    Qinglin Sheng

  4. Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, and National & Local United Engineering Laboratory for Power Batteries, Northeast Normal University, Changchun, Jilin Province, 130024, People’s Republic of China

    Ming Zhou

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  1. Jinqiong Xu
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  2. Xiujuan Qiao
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  3. Yuan Wang
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  4. Qinglin Sheng
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  5. Tianli Yue
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  7. Ming Zhou
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Correspondence to Qinglin Sheng or Tianli Yue.

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Xu, J., Qiao, X., Wang, Y. et al. Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin. Microchim Acta 186, 238 (2019). https://doi.org/10.1007/s00604-019-3339-3

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