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Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid

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Abstract

A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO2@BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO2@BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO2@BSA recovers its fluorescence because MnO2@BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO2 and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.

Schematic presentation of the biomimetic synthesis of blended Au/MnO2@BSA nanoprobes and fluorometric/MR cross-validation dual-modal detection of ascorbic acid.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81571742, 81871399, 81801823), Project funded by China Postdoctoral Science Foundation (1500229020), Shanghai Innovation Program (14ZZ039), Program for Outstanding Young Teachers in Tongji University, and the Fundamental Research Funds for the Central Universities. International Science & Technology Cooperation Program of China (2014DFA33010).

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Author notes

  1. Jiani Yu, Weitao Yang and Shige Xing contributed equally to this work.

Authors and Affiliations

  1. Institute of Photomedicine, Shanghai Skin Disease Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, 200443, People’s Republic of China

    Jiani Yu, Weitao Yang, Jun Wang, Chenyang Xiang & Bingbo Zhang

  2. Chongqing University of Education, Chongqing, 400067, People’s Republic of China

    Jiani Yu

  3. Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing, 100176, People’s Republic of China

    Shige Xing

  4. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China

    Huanxing Han & Pengfei Zhang

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  1. Jiani Yu
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Correspondence to Bingbo Zhang.

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Yu, J., Yang, W., Xing, S. et al. Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid. Microchim Acta 186, 89 (2019). https://doi.org/10.1007/s00604-018-3205-8

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