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Selective, fast and semi-automatic enrichment of nucleosides by using a phenylboronic acid modified hybrid material composed of graphene oxide and melamine sponge

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Abstract

A hybrid material was prepared from graphene oxide and melamine sponge, and modified with phenylboronic acid to obtain a sorbent for the enrichment of the nucleosides cytidine, uridine, inosine, guanosine and adenosine. The loading capacity typically is around 27.8 mg g−1 which is comparable to other sorbents, and highly selectivity for cis-diols is observed even if the concentration of potential interferents is 1500-fold higher. The sorbent was placed in an injector, and the process was operated semiautomatically by using a peristaltic pump. The sorbent is stable and can be re-used six times without decrease in efficiency. It was applied to the selective extraction of the cis-diols (cytidine, uridine, inosine, guanosine, adenosine) from HepG2 cells. It presents good linear between 3 to 5000 μg L−1 and the limits of detection (in HPLC analysis with UV detection) are 1–4 μg L−1. Good recoveries of 85–101% were obtained with spiked HepG2 cells samples, with relative standard deviation of ≤9.9%.

Schematic for the preparation of boronic acid modified graphene oxide/melamine sponge composite for in-syringe solid-phase extraction of nucleosides.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 21375052 and 21575055).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China

    Ting Cheng, Zhijuan Jiao, Xiaoyan Liu & Haixia Zhang

  2. Zhongwei High-Tech Institute of Lanzhou University, Zhongwei, 755000, Ningxia, China

    Haixia Zhang

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  1. Ting Cheng
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  2. Zhijuan Jiao
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  3. Xiaoyan Liu
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  4. Haixia Zhang
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Correspondence to Haixia Zhang.

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Cheng, T., Jiao, Z., Liu, X. et al. Selective, fast and semi-automatic enrichment of nucleosides by using a phenylboronic acid modified hybrid material composed of graphene oxide and melamine sponge. Microchim Acta 185, 348 (2018). https://doi.org/10.1007/s00604-018-2878-3

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