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Cu x O@DNA sphere-based electrochemical bioassay for sensitive detection of Pb2+

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Abstract

The paper describes a one-step synthetic method to chemically reduce cupric sulfate by ascorbic acid in the presence of DNA strands to directly produce Cu x O@DNA spheres. The DNA strands act as template to assist the preparation of Cu x O, and also are capable of specifically binding  Pb(II) ions. The Cu x O@DNA spheres possess high specific surface area and strong bioaffinity. They can be directly employed as platform for detecting Pb2+ sensitively. Electrochemical impedance spectroscopy data showed that the assay exhibits high sensitivity and a wide linear analytical range that extends from 0.1 to 100 nM, and the detection limit is 6.8 pM at a signal-to-noise ratio of 3. The assay is selective, acceptably reproducible, stable, and well feasible for the detection of Pb2+ in blood serum.

Schematic presentation of the preparation of DNA-templated Cu x O spheres (Cu x O@DNA) for use in electrochemical detection of Pb2+. The assay exhibits detection limit of 6.8 pM, high selectivity, acceptable reproducibility, stability, and good applicability for Pb2+ detection.

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Acknowledgements

This work was supported by Programs for the National Natural Science Foundation of China (NSFC: Account Nos. U1604127 and 21601161), Doctoral research foundation of Zhengzhou University of Light Industry (No. 13501050056), and Innovative Technology Team of Henan Province (CXTD2014042).

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Authors and Affiliations

  1. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, China

    Yingpan Song, Chuanpan Guo, Hongfei Ji, Shuai Zhang, Minghua Wang, Linghao He, Donglai Peng & Zhihong Zhang

  2. Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, China

    Yingpan Song, Minghua Wang, Linghao He & Zhihong Zhang

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  1. Yingpan Song
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  2. Chuanpan Guo
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  3. Hongfei Ji
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  4. Shuai Zhang
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  5. Minghua Wang
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  6. Linghao He
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  7. Donglai Peng
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  8. Zhihong Zhang
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Correspondence to Zhihong Zhang.

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Song, Y., Guo, C., Ji, H. et al. Cu x O@DNA sphere-based electrochemical bioassay for sensitive detection of Pb2+. Microchim Acta 185, 186 (2018). https://doi.org/10.1007/s00604-018-2729-2

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  • DOI: https://doi.org/10.1007/s00604-018-2729-2

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