Skip to main content
SpringerLink
Log in

Gold nanoparticles functionalized with Pluronic are viable optical probes for the determination of uric acid

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The authors describe the preparation of gold nanoparticles (AuNPs) coated with poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic F-108) by reducing Au3+ to Au0 using curcumin, a natural and non-toxic food spice, in water of pH ~7 in the presence of F-108 and Ag+ ion. The coated AuNPs display strong resonance Rayleigh scattering (RRS) and fluorescence that results from the functionalization of the gold surface with curcumin and Pluronic F-108. The molar mass of Pluronic F-108 affects the particle size of the AuNPs formed, and small AuNPs are formed when using low molar weight F-108 that was purified by centrifugation or dialysis. The coated AuNPs were employed in an optical method for the determination of uric acid. The combination of uric acid with the AuNPs boosts both the RRS signal and the fluorescence of the AuNPs. However, higher concentrations of uric acid shift the fluorescence peak to shorter wavelengths. The method is simple, and fluorescence, best measured at excitation/emission wavelengths of 425/534 nm, increases linearly in the 50 μM to 50 mM uric acid concentration range, with a 0.14 μM detection limit which is lower than reported for other methods in the literature.

Pluronic F-108 capped gold nanoparticles prepared by reducing Au3+ to Au0 using curcumin can estimate uric acid in 50 μM to 50 mM concentration range.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Sindhu K, Rajaram KA, Sreeram KJ, Rajaram R (2014) Curcumin conjugated gold nanoparticle synthesis and its biocompatibility. RSC Adv 4:1–11

    Article  CAS  Google Scholar 

  2. Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA (2012) The golden age: gold nanoparticles for biomedicine. Chem Soc Rev 41:2740–2779

    Article  CAS  Google Scholar 

  3. Matsui J, Akamatsu K, Hara N, Miyoshi D, Nawafune H, Tamaki K, Sugimoto N (2005) SPR sensor Chip for detection of small molecules\rUsing molecularly imprinted polymer with\rEmbedded gold nanoparticles. Anal Chem 77(13):4282–4285

    Article  CAS  Google Scholar 

  4. Lu Y (2003) A colorimetric lead biosensor using DNAzyme- directed assembly of gold nanoparticles. J Am Chem Soc 125:6642–6643

    Article  Google Scholar 

  5. Zhang S, Wang N, Yu H, Niu Y, Sun C (2005) Covalent attachment of glucose oxidase to an au electrode modified with gold nanoparticles for use as glucose biosensor. Bioelectrochemistry 67(1):15–22

    Article  CAS  Google Scholar 

  6. Daniel MCM, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size related properties and applications toward biology, catalysis and nanotechnology. Chem Rev 104(1):293–346

    Article  CAS  Google Scholar 

  7. Liu H, Hou P, Zhang W, Kim YK, Wu J (2010) The synthesis and characterization of polymer coated FeAu multifonctional nanoparticles. Nanotech 21(33):1–9

    Article  Google Scholar 

  8. Bobulescu IA, Moe OW (2012) Renal transport of uric acid: evolving concepts and uncertainties. Adv Chronic Kidney Dis 19(6):358–371

    Article  Google Scholar 

  9. Kannan P, John SA (2009) Determination of nanomolar uric and ascorbic acids using enlarged gold nanoparticles modified electrode. Anal Biochem 386(1):65–72

    Article  CAS  Google Scholar 

  10. Popa E, Kubota Y, Tryk DA, Fujishima A (2000) Selective voltammetric and amperometric detection of uric acid with oxidized diamond film electrodes. Anal Chem 72(7):1724–1727

    Article  CAS  Google Scholar 

  11. Bravo R, Hsueh CC, Jaramillo A, Brajter-Toth A (1998) Possibilities and limitations in miniaturized sensor design for uric acid. Analyst 123(7):1625–1630

    Article  CAS  Google Scholar 

  12. Chen X, Wu G, Cai Z, Oyama M, Chen X (2014) Advances in enzyme-free electrochemical sensors for hydrogen peroxide, glucose, and uric acid. Microchim Acta 181:689–705

    Article  CAS  Google Scholar 

  13. Kayamori Y, Katayama Y, Urata T (2000) Nonenzymatic elimination of ascorbic acid in clinical samples. Clin Biochem 33:25–29

    Article  CAS  Google Scholar 

  14. Matos RC, Augelli MA, Lago CL, Angnes L (2000) Flow injection analysis-amperometric determination of ascorbic and uric acids in urine using arrays of gold microelectrodes modified by electrodeposition of palladium. Anal Chim Acta 404(1):151–157

    Article  CAS  Google Scholar 

  15. Umasankar Y, Thiagarajan S, Chen SM (2007) Nanocomposite of functionalized multiwall carbon nanotubes with nafion, nano platinum, and nano gold biosensing film for simultaneous determination of ascorbic acid, epinephrine, and uric acid. Anal Biochem 365(1):122–131

    Article  Google Scholar 

  16. Wei Y, Li M, Jiao S, Huang Q, Wang G, Fang B (2006) Fabrication of CeO2 nanoparticles modified glassy carbon electrode and its application for electrochemical determination of UA and AA simultaneously. Electrochim Acta 52(3):766–772

    Article  CAS  Google Scholar 

  17. Dyal C, Nguyen N, Hadden J, Gou L, Murphy CJ (2006) Green synthesis of gold and silver nanoparticles from plant extracts. Chem Mater 231:301–315

    Google Scholar 

  18. Rawat KA, Kailasa SK (2014) Visual detection of arginine, histidine and lysine using quercetin-functionalized gold nanoparticles. Microchim Acta 181(15–16):1917–1929

    Article  CAS  Google Scholar 

  19. Rawat KA, Singhal RK, Kailasa SK (2017) One-pot synthesis of silver nanoparticles using folic acid as a reagent for colorimetric and fluorimetric detections of 6-mercaptopurine at nanomolar concentration. Sens Actuat B: Chemical 249:30–38

    Article  CAS  Google Scholar 

  20. Rawat KA, Kailasa SK (2016) 4-amino nicotinic acid mediated synthesis of gold nanoparticles for visual detection of arginine, histidine, methionine and tryptophan. Sens Actuat B: Chemical 222:780–789

    Article  CAS  Google Scholar 

  21. Moussawi RN, Patra D (2015) Synthesis of au Nanorods through Prereduction with curcumin: preferential enhancement of au Nanorod formation prepared from CTAB-capped over citrate-capped au seeds. J Phys Chem C 119:19458–19468

    Article  CAS  Google Scholar 

  22. El Kurdi R, Patra D (2017) The role of OH- in the formation of highly selective gold nanowires at extreme pH: multi-fold enhancement in the rate of the catalytic reduction reaction by gold nanowires. Phys Chem Chem Phys 19:5077–5090

    Article  Google Scholar 

  23. Mouslmani M, Patra D (2014) Revoking excited state intra-molecular hydrogen transfer by size dependent tailor-made hierarchically ordered nanocapsules. RSC Adv 4:8317

    Article  CAS  Google Scholar 

  24. Patra D, Ahmadieh D, Aridi R (2013) Study on interaction of bile salts with curcumin and curcumin embedded in dipalmitoyl-sn-glycero-3-phosphocholine liposome. Colloids Surf B: Biointerfaces 110:296–304

    Article  CAS  Google Scholar 

  25. Huang X, El-Sayed MA (2010) Gold nanoparticles: optical properties and implementations in cancer diagnosis and photothermal therapy. J Adv Res 1(1):13–28

    Article  Google Scholar 

  26. Boisselier E, Astruc D (2009) Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev 38(6):1759–1782

    Article  CAS  Google Scholar 

  27. Greenberg MA, Hershfield MA (1989) A radiochemical-high-performance liquid chromatographic assay for urate oxidase in human plasma. Anal Biochem 176(2):290–293

    Article  CAS  Google Scholar 

  28. Ghman G (1979) Evaluation of three clinical chemical routine methods for the determination of serum uric acid by mass fragmentography. Clin Chim Acta 95:219–226

    Article  Google Scholar 

  29. Trivedi RC, Rebar L, Berta E, Stong L (1978) New enzymatic method for serum uric acid. Clin Chem 24(11):1908–1911

    CAS  Google Scholar 

  30. Liu J, Li GX, Liu H, Zhou X (1994) Purification and properties of Uricase from Candida Sp. and its Aplplication in uric acid analysis in serum. Appl Biochem Biotechnol 47:57–69

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Financial support provided by Lebanese National Council of Scientific Research (NCSR), Lebanon and American University of Beirut, Lebanon through URB as well as Kamal A. Shair Central Research Laboratory (KAS CRSL) facilities to carry out this work is greatly acknowledged.

Author information

Authors and Affiliations

  1. Department of Chemistry, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, 1107 2020, Lebanon

    Riham El Kurdi & Digambara Patra

Authors
  1. Riham El Kurdi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Digambara Patra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Digambara Patra.

Ethics declarations

The author(s) declare that they have no competing interests.

Electronic supplementary material

ESM 1

(DOCX 779 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

El Kurdi, R., Patra, D. Gold nanoparticles functionalized with Pluronic are viable optical probes for the determination of uric acid. Microchim Acta 185, 185 (2018). https://doi.org/10.1007/s00604-018-2725-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-018-2725-6

Keywords

Search

Navigation