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Recent advances in electrochemical and electrochemiluminescence based determination of the activity of caspase-3

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Abstract

Caspases, especially caspase-3, play a critical role in the intrinsic and extrinsic pathways of apoptosis. In addition, caspase-3 is involved in mental disorders like Alzheimer disease. Any up and down regulation of caspase-3 activity may cause cancer. This review (with 58 references) summarizes recent advances in electrochemical and electrochemiluminescent quantitation of the activity of caspase-3 based on the use of nanomaterials. The nanomaterials and nanolabels are classified in three main subgroups, namely electrochemical signal amplification strategies, amplification based on modified electrodes, and the combination of both modes. The potential of various electrochemical and electrochemiluminescence bioassays is discussed, and methods to circumvent certain limitations are oresented. Finally, current trends in the detection of caspase-3 such as system integration and the application of advanced nanomaterials are discussed.

The review summarizes electrochemical methods for the quantitation of caspase-3 activity based on the use of nanomaterials and of nanomaterial based labels. It contains subsections on electrochemical signal amplification strategies, amplification based on modified electrodes, and the combination of both modes.

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Acknowledgements

The authors gratefully acknowledge for the financial support of this project by Stem Cell Research Center (SCRC) at Tabriz University of Medical Sciences.

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Authors and Affiliations

  1. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Balal Khalilzadeh & Hojjatollah Nozad Charoudeh

  2. Department of Nanochemistry and Nanotechnology Research Center, Urmia University, Urmia, 57154, Iran

    Nasrin Shadjou

  3. Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Hojjatollah Nozad Charoudeh

  4. Research Center for pharrmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad-Reza Rashidi

  5. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad-Reza Rashidi

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  1. Balal Khalilzadeh
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  2. Nasrin Shadjou
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  3. Hojjatollah Nozad Charoudeh
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  4. Mohammad-Reza Rashidi
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Correspondence to Balal Khalilzadeh or Nasrin Shadjou.

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Khalilzadeh, B., Shadjou, N., Charoudeh, H.N. et al. Recent advances in electrochemical and electrochemiluminescence based determination of the activity of caspase-3. Microchim Acta 184, 3651–3662 (2017). https://doi.org/10.1007/s00604-017-2466-y

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