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An optical DNA logic gate based on strand displacement and magnetic separation, with response to multiple microRNAs in cancer cell lysates

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Abstract

A battery of logic gates, “YES”, “AND” and “OR”, are constructed using magnetic beads (MBs) modified by DNA which consists of a substrate strand (S) and a signal strand on which the logic operates. Inputs stemming from micro-RNA (which represent three cancer biomarkers) take the place of signal DNA. The released signal strand self-assembles into the hemin-G-quadruplex complex (DNAzyme) that catalyzes a blue-green dye (ABTS+) from the precursor ABTS. This dye (quantified at a wavelength of 414 nm) represents the output signal for the various logic gates. The method allows quantitative detection of microRNA of three kinds of logic gates in the range of 5 nM–500 nM with detection limits of 3.8 nM, 4.9 nM, 5.4 nM. Boolean logic circuitry is also achieved following the principles of multilevel strand displacement. Based on strand displacement and magnetic separation, this work demonstrates the possibility of designing a logic system using micro-RNA in live cell lysate as inputs, and its potential application in DNA computation and cancer diagnosis.

Schematic representation of a battery of logic gates and the Boolean logic circuitry based on strand displacement and magnetic separation responding to multiple microRNA in cancer cell lysate.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21375070), the Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R39), and the National Basic Research Program of China (2012CB722606).

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Authors and Affiliations

  1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Xiaoting Ji, Haoyuan Lv, Minghui Ma, Binglin Lv & Caifeng Ding

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  1. Xiaoting Ji
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  2. Haoyuan Lv
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  3. Minghui Ma
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  4. Binglin Lv
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  5. Caifeng Ding
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Correspondence to Caifeng Ding.

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Ji, X., Lv, H., Ma, M. et al. An optical DNA logic gate based on strand displacement and magnetic separation, with response to multiple microRNAs in cancer cell lysates. Microchim Acta 184, 2505–2513 (2017). https://doi.org/10.1007/s00604-017-2248-6

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