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Enhanced amperometric immunoassay for the prostate specific antigen using Pt-Cu hierarchical trigonal bipyramid nanoframes as a label

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Abstract

A dual enhancing strategy has been employed to develop a sandwich type of electrochemical immunoassay for the prostate specific antigen (PSA). The signal is enhanced by using Pt-Cu hierarchical trigonal bipyramid nanoframes (HTBNFs) and a composite consisting of Fe3O4 nanoparticles and reduced graphene oxide in polydopamine that serve to capture the primary antibody (Ab1). This nanocomposite shows better electrical conductivity than Fe3O4 and reduced graphene oxide (RGO), respectively, alone. The Pt-Cu HTBNFs were used to label the secondary antibody (Ab2) and act as tags for signal amplification by virtue of their outstanding electrochemical reduction activity towards H2O2. At a working potential of +0.1 V (vs. SCE), the interference by dissolved oxygen can be avoided. This immunoassay is highly sensitive, with a linear range that extends from 0.1 pg⋅mL−1 to 5 ng⋅mL−1 and an ultralow detection limit of 0.03 pg⋅mL−1.

Schematic of the dual amplification strategy in the immunosensor for the prostate specific antigen (PSA) that is based on the use of a first antibody (Ab1) conjugated to a Fe3O4-reduced graphene oxide nanocomposite (Fe3O4-RGO), and of Pt-Cu trigonal bipyramid nanoframes as a label for the second antibody (Ab2).

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Acknowledgements

This work was partially supported by the National Natural Science Foundation of China (no. 21245007 and 81000976).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, and School of Biological Science and Technology, University of Jinan, Jinan, 250022, China

    Lei Jiao, Zonggang Mu, Luyang Miao, Wenwen Du, Qin Wei & He Li

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  1. Lei Jiao
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  2. Zonggang Mu
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  3. Luyang Miao
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  4. Wenwen Du
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  5. Qin Wei
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  6. He Li
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Correspondence to He Li.

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The author(s) declare that they have no competing interests.

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Lei Jiao and Zonggang Mu contributed equally as co-first author.

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Jiao, L., Mu, Z., Miao, L. et al. Enhanced amperometric immunoassay for the prostate specific antigen using Pt-Cu hierarchical trigonal bipyramid nanoframes as a label. Microchim Acta 184, 423–429 (2017). https://doi.org/10.1007/s00604-016-2023-0

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  • DOI: https://doi.org/10.1007/s00604-016-2023-0

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