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Enantioselective electrochemical sensor for R-mandelic acid based on a glassy carbon electrode modified with multi-layers of biotin-loaded overoxidized polypyrrole and nanosheets of reduced graphene oxide

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Abstract

The authors describe an electrochemical method for quantitation of R-mandelic acid (R-MA). A glassy carbon electrode (GCE) was modified by electrochemical deposition of several layers of D-(+)-biotin-loaded overoxidized polypyrrole (OPPy-biot) on nanosheets of reduced graphene oxide. The deposited film was characterized by scanning electron microscopy, differential pulse voltammetry, cyclic voltammetry and electrochemical impedance spectroscopy. The modified GCE is shown to enable stereoselective recognition of R-MA even in the presence of high concentrations of S-mandelic acid (S-MA), best at a voltage of around 1.5 V (vs. Ag/AgCl). The analytical performance of the electrode was studied by differential pulse voltammetry which revealed a linear range that extends over the 5 to 80 mM R-MA concentration range, a 1.5 mM detection limit, and an enanotiospecific characteristic toward R-MA. Under optimum conditions, the method displays high reproducibility and stability. It was applied to the determination of R-MA in a synthetic mixture and no interference was observed by S-MA.

Multi-layered of D-(+)-biotin-loaded overoxidized polypyrrole film on the nanosheets of reduced graphene oxide-modified glassy carbon electrode was fabricated by electrochemical deposition, which are shown to enable enantioselective determination of R-mandelic acid in the presence of high concentration of S-mandelic acid.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, P.O. Box 16315-1618, Tehran, Iran

    Marjan Borazjani, Ehsan Ziaei, Ali Jabbari & Mina Maddah

  2. Department of Marine Living Science, Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, P.O. Box 14155-4781, Tehran, Iran

    Ali Mehdinia

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  1. Marjan Borazjani
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  2. Ali Mehdinia
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Correspondence to Ali Mehdinia.

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Borazjani, M., Mehdinia, A., Ziaei, E. et al. Enantioselective electrochemical sensor for R-mandelic acid based on a glassy carbon electrode modified with multi-layers of biotin-loaded overoxidized polypyrrole and nanosheets of reduced graphene oxide. Microchim Acta 184, 611–620 (2017). https://doi.org/10.1007/s00604-016-1997-y

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  • DOI: https://doi.org/10.1007/s00604-016-1997-y

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