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Bovine serum albumin-stabilized gold nanoclusters as a fluorescent probe for determination of ferrous ion in cerebrospinal fluids via the Fenton reaction

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Abstract

Gold nanoclusters (AuNCs) stabilized with bovine serum albumin were utilized as a fluorescent probe for ferrous ion. The detection scheme is based on the quenching of the fluorescence of the modified AuNCs by hydroxyl radical (•OH) that is generated in the Fenton reaction between Fe(II) and H2O2. Fe(II) can be quantified in the 0.08 to 100 μM concentration range, and the limit of detection is as low as 24 nM. The method also displays good accuracy and high sensitivity when employed to the determination of Fe(II) in rat cerebrospinal fluids (CSFs). When applied to CSFs of a rat model of Alzheimer’s disease, it revealed enhanced levels of Fe(II) compared to a control, thereby showing the important physiological role of iron(II) in this disease.

BSA-stabilized gold nanoclusters (BSA-AuNCs) were utilized for the determination of ferrous ion in rat cerebrospinal fluids. The method is based on the quenching of the fluorescence by hydroxyl radical (•OH) which is generated in the Fenton reaction between Fe(II) and H2O2.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (21105057), Promotive Research Fund for Excellent Young and Middle-aged Scientist of Shandong Province (BS2013SW002) and Innovative Training Project for Undergraduate Students of Shandong Normal University.

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Si Yang, Zhongyao Jiang, Zhenzhen Chen, Lili Tong, Jun Lu & Jiahui Wang

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  1. Si Yang
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  2. Zhongyao Jiang
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  3. Zhenzhen Chen
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  4. Lili Tong
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  5. Jun Lu
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Correspondence to Zhenzhen Chen or Lili Tong.

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Yang, S., Jiang, Z., Chen, Z. et al. Bovine serum albumin-stabilized gold nanoclusters as a fluorescent probe for determination of ferrous ion in cerebrospinal fluids via the Fenton reaction. Microchim Acta 182, 1911–1916 (2015). https://doi.org/10.1007/s00604-015-1525-5

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  • DOI: https://doi.org/10.1007/s00604-015-1525-5

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