Skip to main content
SpringerLink
Log in

An amperometric sensor for nitric oxide based on a glassy carbon electrode modified with graphene, Nafion, and electrodeposited gold nanoparticles

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

This article describes the fabrication of a glassy carbon electrode (GCE) modified with a film containing graphene and gold nanoparticles (AuNPs) that can be used for the determination of nitric oxide (NO) at pH 7.0. A layer of gold nanoparticles was first deposited on the GCE, this followed by coating it with a film of graphene and Nafion. The response of the electrode to NO was studied by cyclic voltammetry and amperometry. A sharp anodic peak was observed at 0.81 V, and the anodic peak current was largely increased compared to a bare GCE or a mono-film modified GCE. A negative shift of the anodic peak potential (by 220 mV) indicates that the composite film has a beneficial effect on ease of the electrochemical oxidation of NO. The electron transfer number was calculated from chronocoulometric data. The amperometric response to NO is linear in the 36 nM to 20 μM concentration range (with a correlation coefficient of 0.9981), and the detection limit is 18 nM (at a S/N of 3). The release of NO from the fish liver homogenate stimulated by L-arginine was studied with this electrode.

A sensitive NO electrochemical sensor was fabricated based on the electrodeposited AuNPs, graphene, and Nafion composite film modified GCE. It possessed a wide calibration range from 36 nM to 20 μM and a low detection limit of 18 nM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Ignarro LJ, Buga GM, Wood KS, Byrns RE, Chaudhuri G (1987) Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc Natl Acad Sci 84:9265–9269

    Article  CAS  Google Scholar 

  2. Moncada S, Palmer RMJ, Higgs EA (1991) Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev 43:109–142

    CAS  Google Scholar 

  3. Koshland DE (1992) NO news is good news. Science 258:1861–1865

    Article  Google Scholar 

  4. Malinski T, Taha Z (1992) Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor. Nature 358:676–678

    Article  CAS  Google Scholar 

  5. Zagal JH, Griveau S, Silva JF, Nyokong T, Bedioui F (2010) Metallophthalo-cyanine-based molecular materials as catalysts for electrochemical reactions. Coord Chem Rev 254:2755–2791

    Article  CAS  Google Scholar 

  6. Chen T, Zamora R, Zuckerbraun B, Billiar TR (2003) Role of nitric oxide in liver injury. Curr Mol Med 3:519–526

    Article  CAS  Google Scholar 

  7. Rees DD, Cellek S, Palmer RMJ, Moncada S (1990) Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effects on vascular tone: an insight into endotoxin shock. Biochem Biophys Res Commun 173:541–547

    Article  CAS  Google Scholar 

  8. Vilakazi SL, Nyokong T (2000) Electrocatalytic properties of vitamin B12 towards oxidation and reduction of nitric oxide. Electrochim Acta 46:453–461

    Article  CAS  Google Scholar 

  9. Wang YZ, Hu SS (2006) A novel nitric oxide biosensor based on electropolymerization poly (Toluidine Blue) film electrode and its application to nitric oxide released in liver homogenate. Biosens Bioelectron 22:10–17

    Article  Google Scholar 

  10. Bedioui F, Griveau S (2013) Electrochemical detection of nitric oxide: assessment of twenty years of strategies. Electroanalysis 25:587–600

    Article  CAS  Google Scholar 

  11. Friedemann MN, Robinson SW, Gerhardt GA (1996) o-Phenylenediamine-modified carbon fiber electrodes for the detection of nitric oxide. Anal Chem 68:2621–2626

    Article  CAS  Google Scholar 

  12. Xu JH, Hu CG, Ji YP, Hu SS (2009) Ultrathin gold film deposited on human hair: derivation from nanoparticles and applications as microsensors. Electrochem Commun 11:764–767

    Article  CAS  Google Scholar 

  13. Kannan P, John SA (2010) Highly sensitive electrochemical determination of nitric oxide using fused spherical gold nanoparticles modified ITO electrode. Electrochim Acta 55:3497–3503

    Article  CAS  Google Scholar 

  14. Dang XP, Hu CG, Wang YK, Hu SS (2011) Gold nanoparticles film grown on quartz fiber and its application as a microsensor of nitric oxide. Sens Actuator B 160:260–265

    Article  CAS  Google Scholar 

  15. Wen W, Chen W, Ren QQ, Hu XY, Xiong HY, Zhang XH, Wang SF, Zhao YD (2012) A highly sensitive nitric oxide biosensor based on hemoglobin-chitosan / grapheme -hexadecyltrimethylammonium bromide nanomatrix. Sens Actuator B 167:444–450

    Article  Google Scholar 

  16. Jayabal S, Viswanathan P, Ramaraj R (2014) Reduced graphene oxide–gold nanorod composite material stabilized in silicate sol–gel matrix for nitric oxide sensor. RSC Adv 4:33541–33548

    Article  CAS  Google Scholar 

  17. Ting SL, Guo CX, Leong KC, Kim DH, Li CM, Chen P (2013) Gold nanoparticles decorated reduced graphene oxide for detecting the presence and cellular release of nitric oxide. Electrochim Acta 111:441–446

    Article  CAS  Google Scholar 

  18. Xu MQ, Wu JF, Zhao GC (2013) Direct electrochemistry of hemoglobin at a graphene gold nanoparticle composite film for nitric oxide biosensing. Sensors 13:7492–7504

    Article  CAS  Google Scholar 

  19. Briza PL, Arben M (2012) Carbon nanotubes and graphene in analytical sciences. Microchim Acta 179:1–16

    Article  Google Scholar 

  20. Kang XH, Wang J, Wu H, Aksay IA, Liu J, Lin YH (2009) Glucose oxidase-graphenechitosan modified electrode for direct electrochemistry and glucose sensing. Biosens Bioelectron 25:901–905

    Article  CAS  Google Scholar 

  21. Huang CC, Bai H, Li C, Shi GQ (2011) A graphene oxide/hemoglobin composite hydrogel for enzymatic catalysis in organic solvents. Chem Commun 47:4962–4964

    Article  CAS  Google Scholar 

  22. Davis D, Guo X, Musavi L, Lin CS, Chen SH, Wu VCH (2013) Gold nanoparticle-modified carbon electrode biosensor for the detection of listeria monocytogenes. Ind Biotech 9:31–36

    Article  CAS  Google Scholar 

  23. El-Deab MS, Sotomura T, Ohsaka T (2005) Size and crystallographic orientation controls of gold nanoparticles electrodeposited on GC electrodes. J Electrochem Soc 152:C1–C6

    Article  CAS  Google Scholar 

  24. Dai X, Nekrassova O, Hyde ME, Compton RG (2004) Anodic stripping voltammetry of arsenic(III) using gold nanoparticle-modified electrodes. Anal Chem 76:5924–5929

    Article  CAS  Google Scholar 

  25. Mahmoud KA, Hrapovic S, Luong JHT (2008) Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode. ACS Nano 2:1051–1057

    Article  CAS  Google Scholar 

  26. Liu H, Penner RM (2000) Size-selective electrodeposition of mesoscale metal particles in the uncoupled limit. J Phys Chem B 104:9131–9139

    Article  CAS  Google Scholar 

  27. Chu L, Han L, Zhang XL (2011) Electrochemical simultaneous determination of nitrophenol isomers at nano-gold modified glassy electrode. J Appl Electrochem 41:687–694

    Article  CAS  Google Scholar 

  28. Wang YZ, Li Q, Hu SS (2005) A multiwall carbon nanotubes film-modified carbon fiber ultramicroelectrode for the determination of nitric oxide radical in liver mitochondria. Bioelectrochemistry 65:135–142

    Article  CAS  Google Scholar 

  29. Butler AR, Williams DLH (1993) The physiological roles of nitric oxide. Chem Soc Rev 22:233–241

    Article  CAS  Google Scholar 

  30. Trevin S, Bedioui F, Devynck J (1996) New electropolymerized nickel porphyrin films. Application to the detection of nitric oxide in aqueous solution. J Electroanal Chem 408:261–265

    Article  Google Scholar 

  31. Lin XY, Ni YN, Kokot S (2013) Glassy carbon electrodes modified with gold nanoparticles for the simultaneous determination of three food antioxidants. Anal Chim Acta 76:54–62

    Article  Google Scholar 

  32. Laviron E (1974) Adsorption autoinhibition and autocatalysis in polarography and linear potential sweep voltammetry. J Electroanal Chem 52:355–393

    Article  CAS  Google Scholar 

  33. Anson FC (1966) Innovations in the study of adsorbed reactants by chronocoulometry. Anal Chem 38:54–57

    Article  CAS  Google Scholar 

  34. Wang YZ, Chen YG, He ZK, Hu SS (2006) A novel electrochemical sensor for the determination of nitric oxide. Chem J Chin Univ 27:43–46

    Article  Google Scholar 

  35. Gerhard GA, Oke AF, Nagy G, Moghaddam B, Adams RN (1984) Nafion-coated electrodes with high selectivity for CNS electrochemistry. Brain Res 290:390–395

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the national Natural Science Foundation of China (No. 31070855) and Technology and Innovation Project of Wuhan Science and Technology Bureau (No.201250499145-9).

Author information

Authors and Affiliations

  1. Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan, China, 430056

    Yazhen Wang

  2. School of Chemistry and Environmental Engineering, Jianghan University, Wuhan, China, 430056

    Yazhen Wang, Bin Song & Junhui Xu

  3. Department of Chemistry, Wuhan University, Wuhan, China, 430079

    Shengshui Hu

Authors
  1. Yazhen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junhui Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shengshui Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yazhen Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, Y., Song, B., Xu, J. et al. An amperometric sensor for nitric oxide based on a glassy carbon electrode modified with graphene, Nafion, and electrodeposited gold nanoparticles. Microchim Acta 182, 711–718 (2015). https://doi.org/10.1007/s00604-014-1379-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-014-1379-2

Keywords

Search

Navigation