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Trans-membrane electron transfer in red blood cells immobilized in a chitosan film on a glassy carbon electrode

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Abstract

We have studied the trans-membrane electron transfer in human red blood cells (RBCs) immobilized in a chitosan film on a glassy carbon electrode (GCE). Electron transfer results from the presence of hemoglobin (Hb) in the RBCs. The electron transfer rate (k s) of Hb in RBCs is 0.42 s−1, and <1.13 s−1 for Hb directly immobilized in the chitosan film. Only Hb molecules in RBCs that are closest to the plasma membrane and the surface of the electrode can undergo electron transfer to the electrode. The immobilized RBCs displayed sensitive electrocatalytic response to oxygen and hydrogen peroxide. It is believed that this cellular biosensor is of potential significance in studies on the physiological status of RBCs based on observing their electron transfer on the modified electrode.

The transmembrane electron transfer rate of Hb in RBCs is slower than hemoglobin molecules directly immobilized on the chitosan film. Only those hemoglobin in RBCs closest to the plasma membrane and electrode could exchange electrons with the electrode. The immobilized RBCs showed sensitive electrocatalytic response to O2 and H2O2.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (81001263, 21175075, 20875051 and 21075070), the Natural Science Foundation of Jiangsu Province (BK2009152, BK2011047), the Universities Natural Science Foundation of Jiangsu Province (10KJB150015), the Application Research Item of Nantong City (BK2011020) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong, 226019, People’s Republic of China

    Chunmei Yu, Li Wang, Zhenkun Zhu, Ning Bao & Haiying Gu

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  1. Chunmei Yu
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  2. Li Wang
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  3. Zhenkun Zhu
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  4. Ning Bao
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  5. Haiying Gu
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Correspondence to Haiying Gu.

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Chunmei Yu and Li Wang contributed equally to this work.

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Yu, C., Wang, L., Zhu, Z. et al. Trans-membrane electron transfer in red blood cells immobilized in a chitosan film on a glassy carbon electrode. Microchim Acta 181, 55–61 (2014). https://doi.org/10.1007/s00604-013-1060-1

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  • DOI: https://doi.org/10.1007/s00604-013-1060-1

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