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Micro-solid phase extraction of ochratoxin A, and its determination in urine using capillary electrophoresis

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Abstract

We describe a simple, environmentally friendly and selective technique for the determination of ochratoxin A (OTA) in urine. It involves (a) the use of a molecularly imprinted polymer as a sorbent in micro-solid-phase extraction in which the sorbent is contained in a propylene membrane envelope, and (b) separation and detection by capillary electrophoresis (CE). Under optimized conditions, response is linear in the range between 50 and 300 ng mL−1 (with a correlation coefficient of 0.9989), relative standard deviations range from 4 to 8 %, the detection limit for OTA in urine is 11.2 ng mL−1 (with a quantification limits of 32.5 ng mL−1) which is lower than those of previously reported methods for solid-phase extraction combined with CE. The recoveries of OTA from urine spiked at levels of 50, 150 and 300 ng mL−1 ranged from 93 to 97 %.

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Acknowledgments

Financial support of the work by a Universiti Sains Malaysia (USM) Postgraduate Research Grant Scheme (USM-RU-PRGS), 1001/PKIMIA/843052 and USM Research Fellowship Program is gratefully acknowledged.

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Authors and Affiliations

  1. School of Chemical Sciences Universiti Sains Malaysia, 11800, Penang, Malaysia

    Tien Ping Lee & Bahruddin Saad

  2. Doping Control Centre Universiti Sains Malaysia, 11800, Penang, Malaysia

    Bahruddin Saad

  3. School of Biological Sciences Universiti Sains Malaysia, 11800, Penang, Malaysia

    Baharuddin Salleh

  4. Unit Kanser MAKNA-USM, Advanced Medical & Dental Institute, Suite 121&141, EUREKA Complex, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Ishak Mat

Authors
  1. Tien Ping Lee
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  2. Bahruddin Saad
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  4. Ishak Mat
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Correspondence to Bahruddin Saad.

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Lee, T.P., Saad, B., Salleh, B. et al. Micro-solid phase extraction of ochratoxin A, and its determination in urine using capillary electrophoresis. Microchim Acta 180, 1149–1156 (2013). https://doi.org/10.1007/s00604-013-1042-3

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  • DOI: https://doi.org/10.1007/s00604-013-1042-3

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