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Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

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Abstract

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic–organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25–30 μmol L−1, and responses are linear up to a concentration of 1 mmol L−1.

Response (fluorescence lifetime) of a novel optical biosensor for biogenic amines (putrescine, cadaverine) determination based on Pisum sativum diamine oxidase immobilized on magnetically responsive chitosan microparticles with entrapped magnetite encapsulated in inorganic–organic polymer ORMOCER® together with ruthenium complex.

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Acknowledgments

This work was supported by the Action COST TD1003 (Bio-inspired nanotechnologies: from concepts to applications), by the OP RD&I grant no. ED0007/01/01 (Centre of the Region Haná for Biotechnological and Agricultural Research) and by the Grant Agency of the Czech Republic (grant No. IAAX08240901).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Palacky University, Slechtitelu 11, 783 71, Olomouc, Czech Republic

    Kristyna Pospiskova & Marek Sebela

  2. Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Academy of Sciences, Na Sadkach 7, 370 05, Ceske Budejovice, Czech Republic

    Ivo Safarik

  3. Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71, Olomouc, Czech Republic

    Ivo Safarik

  4. Department of Protein Biochemistry and Proteomics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Slechtitelu 11, 783 71, Olomouc, Czech Republic

    Marek Sebela

  5. Institute of Chemical Process Fundamentals, Academy of Sciences, Rozvojova 135, 165 02, Prague 6, Czech Republic

    Gabriela Kuncova

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  1. Kristyna Pospiskova
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  2. Ivo Safarik
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  4. Gabriela Kuncova
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Correspondence to Kristyna Pospiskova.

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Pospiskova, K., Safarik, I., Sebela, M. et al. Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer. Microchim Acta 180, 311–318 (2013). https://doi.org/10.1007/s00604-012-0932-0

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  • DOI: https://doi.org/10.1007/s00604-012-0932-0

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