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Mass-amplifying quantum dots in a fluorescence polarization-based aptasensor for ATP

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Abstract

We report on a fluorescence polarization assay for the detection of the target analyte ATP by making use of an aptasensor and of mass-amplifying CdTe-CdS quantum dots. The ATP aptamer was modified with digoxin antigen and hybridized with its complementary DNA that was modified with the CdTe-CdS quantum dots. Following the addition of digoxin antibody, the mass-amplifying aptasensor probe is formed as a result of the immuno reaction. In the presence of ATP, the polarization of fluorescence decreases because the digoxin antibody becomes dissociated due to the recognition of the ATP by the ATP aptamer. Under optimized conditions, the method has a linear response to ATP in the 10 to 350 μM concentration range, and the limit of detection is 3.7 μM. The method combines the specific recognition capability of aptamers with the sensitivity of an immunoreaction. It has good selectivity and sensitivity, and can be used to detect ATP in serum samples.

 

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Acknowledgments

This work has been supported by National Natural Science Foundation of China (No. 21165004, 21163002), the Guangxi Natural Science Foundation of China (2010GXNSFF013001, 0728043, 2012GXNSFBA053022), Innovation Plan in Graduate Education of Guangxi Province (2010106020703M70) and the project of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University), Ministry of Education of China(CMEMR2011-14).

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  1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004, China

    Jianniao Tian, Yuan Wang, Sheng Chen, Yixuan Jiang, Yanchun Zhao & Shulin Zhao

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  1. Jianniao Tian
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  2. Yuan Wang
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  3. Sheng Chen
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Correspondence to Jianniao Tian.

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Tian, J., Wang, Y., Chen, S. et al. Mass-amplifying quantum dots in a fluorescence polarization-based aptasensor for ATP. Microchim Acta 180, 203–209 (2013). https://doi.org/10.1007/s00604-012-0919-x

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  • DOI: https://doi.org/10.1007/s00604-012-0919-x

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