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A three phase dispersive liquid-liquid microextraction technique for the extraction of antibiotics in milk

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Abstract

We have developed a 3-phase method for dispersive liquid-liquid microextraction of ß-lactam antibiotics in milk. Chloroform and acetonitrile serve as the solvents for extraction and disperssion, respectively, where Aliquat 336 is the carrier. An experimental design based on Plackett-Burman and Central composite designs were applied for the screening and optimization of significant parameters in the extraction method. The experimental conditions for extraction were optimized, and the subsequent HPLC assay gave relative standard deviations and detection limits in the range of 4.3–8.5 % and 50–500 μg L-1, respectively. Preconcentration factors are in the range of 80–125.

We have developed a 3-phase method for dispersive liquid-liquid microextraction of ß-lactam antibiotics in milk. Chloroform and acetonitrile serve as the solvents for extraction and disperssion, respectively, where Aliquat 336 is the carrier. An experimental design based on Plackett-Burman and Central composite designs were applied for the screening and optimization of significant parameters in the extraction method.

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Authors and Affiliations

  1. Department of Chemistry, Shahid Beheshti University, G. C., P. O. Box: 1983963113, Evin, Tehran, Iran

    Laleh Adlnasab & Homeira Ebrahimzadeh

  2. Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran

    Yadollah Yamini

Authors
  1. Laleh Adlnasab
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  2. Homeira Ebrahimzadeh
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  3. Yadollah Yamini
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Correspondence to Homeira Ebrahimzadeh.

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Adlnasab, L., Ebrahimzadeh, H. & Yamini, Y. A three phase dispersive liquid-liquid microextraction technique for the extraction of antibiotics in milk. Microchim Acta 179, 179–184 (2012). https://doi.org/10.1007/s00604-012-0843-0

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  • DOI: https://doi.org/10.1007/s00604-012-0843-0

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