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Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins

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Abstract

Bioactivity of proteins is evaluated to test the adverse effects of nanoparticles interjected into biological systems. Surface plasmon resonance (SPR) spectroscopy detects binding affinity that is normally related to biological activity. Utilizing SPR spectroscopy, a concise testing matrix is established by investigating the adsorption level of bovine serum albumin (BSA) and anti-BSA on the surface covered with 11-mercaptoundecanoic acid (MUA); magnetic nanoparticles (MNPs) and single-walled carbon nanotubes (SWCNTs), respectively. The immunoactivity of BSA on MNPs and SWCNT decreased by 18 % and 5 %, respectively, compared to that on the gold film modified with MUA. This indicates that MNPs cause a considerable loss of biological activity of adsorbed protein. This effect can be utilized for practical applications on detailed biophysical research and nanotoxicity studies.

Schematic diagram of Ab-Ag interaction on MNPs confined Au surface (left) and SPR study on the immunoactivity of BSA adsorbed on MNPs (right).

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (Grant Nos. 31100560, 81172503), Innovation Program of the Shanghai Municipal Education Commission (11YZ07) and by SRF for ROCS, SEM and the Leading Academic Discipline Project of Shanghai Municipal Education Commission (J50108).

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Authors and Affiliations

  1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, 200444, China

    Qiaohan Mei, Yangyang Chen, Jiang Hong & Hongxia Chen

  2. Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

    Xiaorong Ding & Yongmei Yin

  3. Department of Nanomedical Engineering, Pusan National University, Miryang, 627-706, Republic of Korea

    Kwangnak Koh & Jaebeom Lee

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  1. Qiaohan Mei
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  2. Xiaorong Ding
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  3. Yangyang Chen
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  4. Jiang Hong
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  5. Kwangnak Koh
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  6. Jaebeom Lee
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  7. Hongxia Chen
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  8. Yongmei Yin
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Correspondence to Hongxia Chen or Yongmei Yin.

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Mei, Q., Ding, X., Chen, Y. et al. Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins. Microchim Acta 178, 301–307 (2012). https://doi.org/10.1007/s00604-012-0837-y

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  • DOI: https://doi.org/10.1007/s00604-012-0837-y

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