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Determination of some trace elements by flame atomic absorption spectrometry after preconcentration and separation by Escherichia coli immobilized on multiwalled carbon nanotubes

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Abstract

We have immobilized living and non-living Escherichia coli (E. coli) bacteria on multiwalled carbon nanotubes (MWCNT) and used such materials as a biosorbent for the separation and preconcentration of copper, cobalt, cadmium and nickel prior to their determination by flame atomic absorption spectrometry (FAAS). E. coli bacteria cells were mixed with MWCNTs in a 1:1 ratio, dried and placed at the tip of a 50-mL syringe. The ions were retained on the sorbent and then eluted by drawing and ejecting back the sample (or standard solution) and an eluent, respectively. The effects of various experimental parameters on the sorption and elution were investigated. The analytes were quantitatively retained (at pH values of 7) and eluted (with 0.5 M nitric acid) with high precision, the RSD being <5%. The performances of the new sorbents were compared using certified reference materials. The sorbent modified with living E. coli has a higher adsorption capacity and displays somewhat better recoveries compared to sorbent based on non-living E. coli. Both sorbents were successfully used for the separation and preconcentration of copper, cobalt, cadmium and nickel prior to their determination by flame atomic absorption spectrometry.

SEM photograph of (a) living E. coli immobilized on MWCNT and (b) non-living E. coli immobilized on MWCNT

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Authors and Affiliations

  1. Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey

    Nihat Aydemir, Asli Baysal & Süleyman Akman

  2. Scientific and Technical Research Council of Turkey (TUBITAK), National Metrology Institute, PK 54 41470, Gebze, Kocaeli, Turkey

    Nilgun Tokman

  3. Faculty of Science & Letters, Department of Molecular Biology and Genetics, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey

    Alper Tunga Akarsubasi

Authors
  1. Nihat Aydemir
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  2. Nilgun Tokman
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  3. Alper Tunga Akarsubasi
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  4. Asli Baysal
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  5. Süleyman Akman
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Correspondence to Süleyman Akman.

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Aydemir, N., Tokman, N., Akarsubasi, A.T. et al. Determination of some trace elements by flame atomic absorption spectrometry after preconcentration and separation by Escherichia coli immobilized on multiwalled carbon nanotubes. Microchim Acta 175, 185–191 (2011). https://doi.org/10.1007/s00604-011-0668-2

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  • DOI: https://doi.org/10.1007/s00604-011-0668-2

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