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Application of ultrasound-assisted ionic liquid dispersive liquid-phase microextraction followed high-performance liquid chromatography for the determination of fungicides in red wine

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Abstract

A new method was developed for the determination of fungicides in red wine using ultrasound-assisted ionic liquid dispersive liquid–phase microextraction followed by high-performance liquid chromatography. The ionic liquid, 1-hexyl-3-methylimidazolium hexafluorophosphate (IL) was quickly disrupted by ultrasonication and dispersed in wine as fine droplets. At this stage, the analytes were extracted into the fine droplets of IL. After centrifugation, the concentration of the enriched fungicides in the sedimented phase was determined. Extraction conditions including the type of extraction solvent, the extraction solvent volume, ultrasonication time, centrifugation time and sample pH were optimized. The performance of the method was studied in terms of linearity, precision, and recovery. Quantitative recoveries (>70%) except for pyrimethanil were obtained, and method precision was also satisfactory (RSD < 10%). Enrichment factors range from 100 to 200, and the limits of detection are at the low μg per liter level for most of the target compounds.

The typical chromatograms of spiking at the concentration level 50 μgL−1 of each analytes in a red wine. (1) pyrimethanil (2) fenarimol (3) epoxiconazole (4) kresoxim-methyl (5) cypronidil (6) prochloraz (7) diniconazole

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Acknowledgment

The project was sponsored by 40th Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry and DR Research Foundation of Hebei North University, PRC.

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Authors and Affiliations

  1. China Agricultural University, Beijing, 100193, the People’s Republic of China

    Suli Wang, Liping Ren, Yanjun Xu & Fengmao Liu

  2. Hebei North University, Zhangjiakou, 075131, the People’s Republic of China

    Suli Wang

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  1. Suli Wang
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  2. Liping Ren
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  3. Yanjun Xu
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  4. Fengmao Liu
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Correspondence to Fengmao Liu.

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Wang, S., Ren, L., Xu, Y. et al. Application of ultrasound-assisted ionic liquid dispersive liquid-phase microextraction followed high-performance liquid chromatography for the determination of fungicides in red wine. Microchim Acta 173, 453–457 (2011). https://doi.org/10.1007/s00604-011-0577-4

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  • DOI: https://doi.org/10.1007/s00604-011-0577-4

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