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Direct electrochemistry of hemoglobin immobilized on CdS:Mn nanoparticles

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Abstract

Hemoglobin (Hb) was immobilized on CdS:Mn nanoparticles (NPs) capped with citrate to obtain an amperometric biosensor for determination of hydrogen peroxide (H2O2). The NPs were prepared from aqueous solution via a precipitation method. The UV-vis spectra of the film with Hb on the NPs and of free Hb solutions suggested that Hb retained its bioactivity and native conformation in the film. In buffer of pH 7.0, the sensor film exhibited a pair of quasi-reversible redox peaks originating from the Hb-heme Fe(III)/Fe(II) redox couple. The formal potential of Hb varied linearly with the increase of pH from 3.0 to 9.0 with a slope of −46 mV·pH−1, this indicating that one proton participates in the redox reaction. The electron transfer rate constant (ks) is 2.2 s−1, suggesting that the NPs provide a benign microenvironment for Hb to undergo direct electron transfer. Despite the absence of an electron mediator, the biosensor displays an analytical performance which is better than any others described in previous reports. Hence, the NPs may provide a simple and alternative approach towards electrochemical biosensors.

The CdS:Mn NPs capped with citrate may provide a benign microenvironment for Hb to undergo direct electron transfer so that the electrochemical detection could be improved.

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Acknowledgement

This work was financially supported by the National Natural Science Foundation of China (Grant numbers: 20675042; 20875051; 21075070; 81001263), the Natural Science Foundation of Jiangsu Province (Grant number: BK2009152).

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Authors and Affiliations

  1. Institute of Nautical Medicine, Nantong University, Nantong, 226001, People’s Republic of China

    Zhong-Qin Pan & Hai-Ying Gu

  2. Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, 226019, People’s Republic of China

    Zhong-Qin Pan, Hong Fan, Chuan-Guo Shi, Ning Bao, Chun-Mei Yu & Hai-Ying Gu

  3. School of Public Health, Nantong University, Nantong, 226019, People’s Republic of China

    Hong Fan, Ning Bao, Chun-Mei Yu & Hai-Ying Gu

  4. College of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, People’s Republic of China

    Chuan-Guo Shi

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  1. Zhong-Qin Pan
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Correspondence to Hai-Ying Gu.

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Fig. S1

EDS spectrum of CdS:Mn NPs capped with citrate. (DOC 60 kb)

Fig. S2

The UV-vis spectra of (a) CdS:Mn-CS modified ITO, (b) Hb in pH 6.0 phosphate buffer solution and (c) Hb-CdS:Mn-CS modified ITO. (DOC 450 kb)

Fig. S3

Cyclic voltammograms of Hb-CdS:Mn-CS modified GCE in pH 7.0 phosphate buffer solution at various scan rates: 50, 100, 150, 200, 250, 300, 350, 400 mV·s−1 (a-h). Inset: plots of cathodic and anodic peak currents vs. scan rate. (DOC 585 kb)

Fig. S4

CVs of Hb-CdS:Mn-CS-GCE in pH 7.0 phosphate buffer solution at 100 mV·s−1 in the presence of (a) 0, (b) 5 μ M H2O2, (c) 10 μ M H2O2 and (d)15 μ M H2O2. (DOC 313 kb)

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Pan, ZQ., Fan, H., Shi, CG. et al. Direct electrochemistry of hemoglobin immobilized on CdS:Mn nanoparticles. Microchim Acta 173, 277–283 (2011). https://doi.org/10.1007/s00604-011-0559-6

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  • DOI: https://doi.org/10.1007/s00604-011-0559-6

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