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Nanogold hollow microsphere-based electrochemical immunosensor for the detection of ferritin in human serum

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Abstract

A simple and sensitive electrochemical immunosensor was designed for the determination of ferritin (FeAg, as a model) in human serum by using a nanogold hollow microsphere (NGHS)-functionalized interface as an immunosensing probe. The presence of NGHSs provided a congenial microenvironment with large surface coverage for the immobilization of ferritin antibodies (FeAb). The formation of the antibody-antigen complex by a simple one-step immunoreaction between the immobilized FeAb and FeAg in sample solution introduced a change of membrane charge on the surface of the immunosensing probe. Thus, local potential variations could be detected by potentiometry. Compared to conventional gold nanoparticle probes, the NGHS-modified immunosensors exhibit wide dynamic range from 1.0 to 500 ng·mL−1 toward FeAg, with a detection limit of 0.1 ng·mL−1 (at 3σ). The selectivity, reproducibility and stability of the immunosensors are acceptable. The assay was evaluated with clinical serum specimens (n = 25), and excellent correspondence with the reference enzyme-linked immunosorbent assay method was obtained.

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Acknowledgments

The authors greatly acknowledge the funding support of the National Natural Science Foundation of China (Project Nos. 20345006 and 20575043).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 215006, China

    Xiuling Wang, Guanhong Tao & Yuanhua Meng

  2. School of Chemistry and Chemical Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China

    Xiuling Wang

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  1. Xiuling Wang
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  2. Guanhong Tao
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  3. Yuanhua Meng
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Correspondence to Guanhong Tao.

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Wang, X., Tao, G. & Meng, Y. Nanogold hollow microsphere-based electrochemical immunosensor for the detection of ferritin in human serum. Microchim Acta 167, 147 (2009). https://doi.org/10.1007/s00604-009-0225-4

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  • DOI: https://doi.org/10.1007/s00604-009-0225-4

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