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Nanosilver and DNA-functionalized immunosensing probes for electrochemical immunoassay of alpha-fetoprotein

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Abstract

A simple and sensitive electrochemical immunosensor for a one-step immunoassay for alpha-fetoprotein (AFP) was designed using silver nanoparticles and double-stranded DNA as matrices. The detection was based on the change in the electron transfer resistance before and after the antigen-antibody reaction by using electrochemical impedance spectroscopy. Under optimal conditions, the resistance shift of the immunosensor is proportional to the AFP concentration in the range 3.5 –360 ng·mL−1 with a detection limit of 1.5 ng·mL−1 (at 3σ). The immunosensor exhibits high sensitivity, good reproducibility and stability. Results obtained for clinical serum samples by the immunosensor are in accordance with those determined by spectrophotometric enzyme-linked immunosorbent assays.

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Acknowledgements

Supports from the NNSF of China and the NSF of Yuzhong District, Chongqing, China are gratefully acknowledged.

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Authors and Affiliations

  1. Trauma Centre of PLA, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Shijin Sun, Yuanzhang Yao, Tao Wang, Yingcai Li, Xiaolin Ma & Lianyang Zhang

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  1. Shijin Sun
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  2. Yuanzhang Yao
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  3. Tao Wang
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  4. Yingcai Li
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  5. Xiaolin Ma
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  6. Lianyang Zhang
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Corresponding authors

Correspondence to Xiaolin Ma or Lianyang Zhang.

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Sun, S., Yao, Y., Wang, T. et al. Nanosilver and DNA-functionalized immunosensing probes for electrochemical immunoassay of alpha-fetoprotein. Microchim Acta 166, 83–88 (2009). https://doi.org/10.1007/s00604-009-0174-y

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  • DOI: https://doi.org/10.1007/s00604-009-0174-y

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