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Determination of cobalt in biological materials by RNAA via induced short-lived 60mCo

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Abstract.

A procedure is described for the determination of cobalt in biological materials, based on post-irradiation separation radiochemical neutron activation analysis (RNAA) using the short-lived induced radionuclide 60mCo. 60mCo has been rarely used as an indicator radionuclide in NAA due to low concentrations usually found in biological materials and due to its short-half life (t 1/2=10.5 min). Using a procedure based on dissolution of the irradiated sample, precipitation of Co with sodium diethyldithiocarbamate, extraction of the complexes formed into toluene, purification and counting by gamma spectrometry, Co was determined in biological materials at ng g−1 levels. For evaluation of gamma spectra, the Hypermet-PC and HyperLab 2002 Gamma Spectroscopy systems were used. Differences between these software programs are discussed. The results of the analyzed biological materials (reference materials), together with the identified uncertainty sources, are presented. The RNAA method allows rapid, robust and simple separation of short-lived 60mCo from neutron irradiated biological samples in high and reproducible yield (83%), with adequate sensitivity for routine use, reducing the time necessary for the irradiation, processing and measurement in comparison to classical NAA using 60Co, thus retaining the important advantage of NAA as a blank free technique.

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References

  • M De Boeck M Kirsch-Volders D Lison (2003) ArticleTitleCobalt and antimony: genotoxicity and carcinogenicity Mutat Res 533 135 Occurrence Handle1:CAS:528:DC%2BD3sXpt1erurc%3D

    CAS  Google Scholar 

  • L Husáková A Bobrowski J Šrámková A Królicka K Vytřas (2005) ArticleTitleCatalytic adsorptive stripping voltammetry versus electrothermal atomic absorption spectrometry in the determination of trace cobalt and chromium in human urine Talanta 66 999 Occurrence Handle10.1016/j.talanta.2005.01.003

    Article  Google Scholar 

  • M Korolczuk A Moroziewicz M Grabarczyk K Paluszek (2005) ArticleTitleDetermination of traces of cobalt in the presence of nioxime and cetyltrimethylammonium bromide by adsorptive stripping voltammetry Talanta 65 1003 Occurrence Handle10.1016/j.talanta.2004.08.033 Occurrence Handle1:CAS:528:DC%2BD2MXoslCkug%3D%3D

    Article  CAS  Google Scholar 

  • M Korolczuk K Tyszczuk M Grabarczyk (2005) ArticleTitleAdsorptive stripping voltammetry of nickel and cobalt at in situ plated lead film electrode Electrochem Commun 7 1185 Occurrence Handle10.1016/j.elecom.2005.08.022 Occurrence Handle1:CAS:528:DC%2BD2MXht1alu7%2FL

    Article  CAS  Google Scholar 

  • P Kajič I Milošev B Pihlar V Pišot (2003) ArticleTitleDetermination of trace cobalt concentrations in human serum by adsorptive stripping voltammetry J Trace Elem Med Biol 17 153 Occurrence Handle10.1016/S0946-672X(03)80019-6

    Article  Google Scholar 

  • E A Hutton B Ogorevc S B Hočevar M R Smyth (2006) ArticleTitleBismuth film microelectrode for direct voltammetric measurement of trace cobalt and nickel in some simulated and real body fluid samples Anal Chim Acta 557 57 Occurrence Handle10.1016/j.aca.2005.10.003 Occurrence Handle1:CAS:528:DC%2BD28XotFyjtg%3D%3D

    Article  CAS  Google Scholar 

  • V A Lemos J S Santos L S Nunes M B de Carvalho P X Baliza R T Yamaki (2003) ArticleTitleAmberlite XAD-2 functionalized with Nitroso R salt: synthesis and application in an online system for preconcentration of cobalt Anal Chim Acta 494 87 Occurrence Handle10.1016/j.aca.2003.08.002 Occurrence Handle1:CAS:528:DC%2BD3sXns12ntLs%3D

    Article  CAS  Google Scholar 

  • I Komjarova R Blust (2006) ArticleTitleComparison of liquid–liquid extraction, solid-phase extraction and co-precipitation preconcentration methods for the determination of cadmium, copper, nickel, lead and zinc in seawater Anal Chim Acta 576 221 Occurrence Handle10.1016/j.aca.2006.06.002 Occurrence Handle1:CAS:528:DC%2BD28XotlWksro%3D

    Article  CAS  Google Scholar 

  • A S Ribeiroa M A Vieiraa A F da Silvaa D L G Borgesa B Welza U Heitmannb A J Curtius (2005) ArticleTitleDetermination of cobalt in biological samples by line-source and high-resolution continuum source graphite furnace atomic absorption spectrometry using solid sampling or alkaline treatment Spectrochimica Acta Part B 60 693 Occurrence Handle10.1016/j.sab.2005.01.002

    Article  Google Scholar 

  • Greenberg R R (2003) Intrinsic quality assurance aspects of neutron activation analysis. Conference and Symposium Paper. International Conference on Isotopic and Nuclear Analytical Techniques for Health and Environment, IAEA

  • M Navarrete L Cabrera T Martínez (2004) ArticleTitleUse of activated carbon as pre-separation agent in NAA of selenium, cobalt and iodine J Radioanal Nucl Chem 262 195 Occurrence Handle10.1023/B:JRNC.0000040874.36032.30 Occurrence Handle1:CAS:528:DC%2BD2cXntlCjsLc%3D

    Article  CAS  Google Scholar 

  • Bate L C, Leddicotte G W (1961) Radiochemistry of cobalt. Nuclear Science Series. National Academy of Sciences/National Research Council, U.S. Atomic Energy Commission

  • J Kučera G V Iyengar Z Řanda R M Parr (2004) ArticleTitleDetermination of iodine in Asian diets by epithermal and radiochemical neutron activation analysis J Radioanal Nucl Chem 259 505 Occurrence Handle10.1023/B:JRNC.0000020927.30825.bd

    Article  Google Scholar 

  • Technical report series No. 197 (1980) Elemental analysis of biological materials. Current problems and techniques with special reference to trace elements, IAEA. Vienna

  • The IAEA Database of Natural Matrix Reference Materials (NMRM), http://www.iaea.org/programmes/aqcs/

  • NIST, http://ts.nist.gov/measurementservices/referencematerials/index.cfm

  • EURACHEM/CITAC Guide CG 4 (2000) Quantifying uncertainty in analytical measurement, QUAM: 2000.1, http://www.eurachem.org/

  • J Kučera P Bode V Štěpánek (2000) ArticleTitleThe 1993 ISO guide to the expression of uncertainty in measurement applied to NAA J Radioanal Nucl Chem 245 115 Occurrence Handle10.1023/A:1006760726572

    Article  Google Scholar 

  • A R Byrne C Camara-Rica R Cornelis J J M de Goeij G V Iyengar G Kirkbright G Knapp R M Parr M Stoeppler (1987) ArticleTitleResults of a co-ordinated research programme to improve the certification of IAEA milk powder A 11 and animal muscle H 4 for eleven “difficult” trace elements Fresenius Z Anal Chem 326 723 Occurrence Handle10.1007/BF00473540 Occurrence Handle1:CAS:528:DyaL2sXktVenuro%3D

    Article  CAS  Google Scholar 

  • R S Dybczyński B Danko H Polkowska-Motrenko Z Samczyński (2007) ArticleTitleRNAA in metrology: a highly accurate (definitive) method Talanta 71 529 Occurrence Handle10.1016/j.talanta.2006.04.021

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Environmental Sciences, Jozef Stefan Institute, Ljubljana, Slovenia

    Urška Repinc & Ljudmila Benedik

  2. European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel, Belgium

    Ljudmila Benedik

  3. Faculty for chemistry and chemical technology, University of Ljubljana, Ljubljana, Slovenia

    Boris Pihlar

Authors
  1. Urška Repinc
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  2. Ljudmila Benedik
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  3. Boris Pihlar
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Correspondence to Urška Repinc.

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Correspondence: Urška Repinc, Department of Environmental Sciences, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia

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Repinc, U., Benedik, L. & Pihlar, B. Determination of cobalt in biological materials by RNAA via induced short-lived 60mCo. Microchim Acta 162, 141–146 (2008). https://doi.org/10.1007/s00604-008-0939-8

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  • DOI: https://doi.org/10.1007/s00604-008-0939-8

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