Abstract
An electrochemical biosensor was fabricated by covalent modification of 5-hydroxytryptophan (5-HTP) on the surface of glassy carbon electrode (GCE). The electrode, denoted as 5-HTP/GCE, was characterized by X-ray photoelectron spectroscopy, cyclic voltammetry and differential pulse voltammetry. For comparison, tryptophan modified GCE (TRP/GCE) and serotonin modified GCE (5-HT/GCE) were prepared by the same method. It was found that electrocatalytic ability of these electrodes was in the order of 5-HTP/GCE > TRP/GCE > 5-HT/GCE for the oxidation of dopamine (DA) and 5-HT. The sensor was effective to simultaneously determine DA and 5-HT in a mixture. It can resolve the overlapping anodic peaks into two well-defined voltammetric peaks at 0.24 and 0.39 V (versus SCE). The linear response is in the range of 5.0 × 10−7–3.5 × 10−5 mol L−1 with a detection limit of 3.1 × 10−7 mol L−1 for DA, and in the range of 5.0 × 10−6–3.5 × 10−5 mol L−1 with a detection limit of 1.7 × 10−6 mol L−1 for 5-HT (s/n = 3), respectively.
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Acknowledgements
This work was supported financially by the Natural Science Foundation of Educational Department of Anhui Province (No. 2006kj039a), the National Natural Science Foundation of China (No. 20575001), and a Project for the Author of Excellent Teacher of Anhui Province.
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Supplementary Material
The covalent modification process between 5-HTP and GCE) and the XPS of 5-HTP/GCE in the N (1s) region. This materials are available in electronic form via the homepage of MCA.
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Li, Y., Huang, X., Chen, Y. et al. Simultaneous determination of dopamine and serotonin by use of covalent modification of 5-hydroxytryptophan on glassy carbon electrode. Microchim Acta 164, 107–112 (2009). https://doi.org/10.1007/s00604-008-0040-3
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DOI: https://doi.org/10.1007/s00604-008-0040-3