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Flow injection fluorimetric determination of L-dopa and dopamine based on a photochemical inhibition process

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Abstract.

An automatic procedure has been developed for the determination of L-dopa and dopamine. It is based on the strong inhibition of the photochemical reaction between thionine blue and ethylenediamine-tetraacetic acid by these catecholamines. The proposed flow-injection method allows for the fluorimetric determination of L-dopa in the range of 1.9–98 µg mL−1 and that of dopamine in the range of 1.9–75 µg mL−1 with a sampling frequency of 35 samples h−1. The method was applied successfully to the determination of dopamine in pharmaceutical preparations.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071, Murcia, Spain

    Tomás Pérez-Ruiz, Carmen Martínez Lozano, Virginia Tomás & Encarna Ruiz

Authors
  1. Tomás Pérez-Ruiz
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  2. Carmen Martínez Lozano
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  3. Virginia Tomás
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  4. Encarna Ruiz
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Correspondence to Tomás Pérez-Ruiz.

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Pérez-Ruiz, T., Martínez Lozano, C., Tomás, V. et al. Flow injection fluorimetric determination of L-dopa and dopamine based on a photochemical inhibition process. Microchim Acta 158, 299–305 (2007). https://doi.org/10.1007/s00604-006-0710-y

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  • DOI: https://doi.org/10.1007/s00604-006-0710-y

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