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Lung function early after lung transplantation is correlated with the frequency of regulatory T cells

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Abstract

Purposes

Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period.

Methods

This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS.

Results

Treg-specific markers (Foxp3, CD127lo, and CTLA4) in the CD25+CD4+ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4+CD25+ T cells than in CD4+CD25− T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients.

Conclusions

The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.

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Acknowledgment

We thank Dr. Yuko Ohno of the Department of Health Promotion Science, Osaka University Graduate School of Medicine, Division of Health Sciences, for her critical review of the manuscript and advice on statistical issues.

Conflict of interest

Tomoyuki Nakagiri and his co-authors have no conflict of interest.

Author information

Authors and Affiliations

  1. Division of Thoracic and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany

    Tomoyuki Nakagiri, Gregor Warnecke, Murat Avsar, Stefanie Thissen, Bianca Kruse, Christian Kühn, Petra Ziehme, Ann-Kathrin Knöfel, Nodir Madrahimov, André R. Simon, Axel Haverich & Martin Strüber

  2. Department of General Thoracic Surgery, Osaka University Graduate School of Medicine, Osaka, Japan

    Tomoyuki Nakagiri & Meinoshin Okumura

  3. Division of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan

    Yoshiki Sawa

  4. Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany

    Jens Gottlieb

Authors
  1. Tomoyuki Nakagiri
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  2. Gregor Warnecke
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  3. Murat Avsar
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  4. Stefanie Thissen
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  8. Ann-Kathrin Knöfel
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  11. Yoshiki Sawa
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  12. Jens Gottlieb
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  13. André R. Simon
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  14. Axel Haverich
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  15. Martin Strüber
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Corresponding author

Correspondence to Martin Strüber.

Additional information

This work was supported by a grant from Osaka University and by the DFG (SFB 738).

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Nakagiri, T., Warnecke, G., Avsar, M. et al. Lung function early after lung transplantation is correlated with the frequency of regulatory T cells. Surg Today 42, 250–258 (2012). https://doi.org/10.1007/s00595-011-0087-3

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  • DOI: https://doi.org/10.1007/s00595-011-0087-3

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