Skip to main content

Advertisement

SpringerLink
Log in

Histological Features of Human Papilloma Virus 16 and Its Association with the Development and Progression of Anal Squamous Cell Carcinoma

  • Published:
Surgery Today Aims and scope Submit manuscript

Abstract

Purpose

To investigate the development of anal squamous cell carcinoma (SCC) and the expression patterns of human papillomavirus (HPV).

Methods

We performed typing of HPV-DNA using a polymerase chain reaction (PCR), and amplified the Duchenne muscular dystrophy (DMD) genes simultaneously, to investigate the tumor DNA state. The expression patterns of HPV in the cancer cell nuclei was investigated by in situ hybridization (ISH) using HPV probes.

Results

Amplification of DMD genes was confirmed in 8 of 20 patients with anal SCC, suggesting that tumor DNA was preserved in these patients. In seven of these eight patients, only HPV16 was detected by both PCR and ISH, suggesting HPV16-induced carcinogenesis. In two patients with carcinoma in situ (CIS), the cancer cells showed only a diffuse pattern (DP), and in two patients with invasive cancer, the cancer cell showed only an oligo-dot pattern (OP). In one patient with lesions ranging from CIS to invasive cancer, the histologic features varied in each area, from DP to OP. This change originated in the deep part of the microinvasive area.

Conclusions

These findings show that HPV16 infection is closely involved in the development of anal SCC and suggest that the change in the genome occurs at the stage of microinvasive cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H Ikenberg L Gissmann G Gross EI Grussendorf-Conen H zur Hausen (1983) ArticleTitleHuman papillomavirus type-16-related DNA in genital Bowen's disease and in bowenoid papulosis Int J Cancer 32 563–5 Occurrence Handle6315601 Occurrence Handle1:STN:280:BiuD28%2FjvVI%3D

    PubMed  CAS  Google Scholar 

  2. M Perez-Ayala F Ruiz-Cabello F Esteban A Concha M Redondo MR Oliva et al. (1990) ArticleTitlePresence of HPV 16 sequence in laryngeal carcinomas Int J Cancer 46 8–11 Occurrence Handle2163992 Occurrence Handle1:CAS:528:DyaK3MXnsF2iug%3D%3D

    PubMed  CAS  Google Scholar 

  3. K Anwar H Naiki K Nakakuki M Inuzuka (1992) ArticleTitleHigh frequency of human papillomavirus infection in carcinoma of the urinary bladder Cancer 70 1967–73 Occurrence Handle1326398 Occurrence Handle1:STN:280:By2H3crgvVM%3D Occurrence Handle10.1002/1097-0142(19921001)70:7<1967::AID-CNCR2820700726>3.0.CO;2-8

    Article  PubMed  CAS  Google Scholar 

  4. MD Lai MJ Luo JE Yao PH Chen (1998) ArticleTitleAnal cancer in Chinese: human papillomavirus infection and altered expression of p53 World J Gastroenterol 4 298–302 Occurrence Handle11819303 Occurrence Handle1:CAS:528:DyaK1MXht1GlsL8%3D

    PubMed  CAS  Google Scholar 

  5. M Indinnimeo C Cicchini A Stazi E Giarnieri D French MR Limiti et al. (1999) ArticleTitleHuman papillomavirus infection and p53 nuclear overexpression in anal canal carcinoma J Exp Clin Cancer Res 18 47–52 Occurrence Handle10374676 Occurrence Handle1:STN:280:DyaK1MzgtFaisA%3D%3D

    PubMed  CAS  Google Scholar 

  6. H Yoshikawa T Kawana K Kitagawa M Mizuno H Yoshikura A Iwamoto (1991) ArticleTitleDetection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers Jpn J Cancer Res 82 524–31 Occurrence Handle1648051 Occurrence Handle1:CAS:528:DyaK3MXmslCrsbk%3D

    PubMed  CAS  Google Scholar 

  7. JS Chamberlain RA Gibbs JE Ranier PN Nguyen CT Caskey (1988) ArticleTitleDeletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification Nucleic Acids Res 16 11141–56 Occurrence Handle3205741 Occurrence Handle1:CAS:528:DyaL1MXht12rt7Y%3D

    PubMed  CAS  Google Scholar 

  8. ER Unger LR Budgeon D Myerson DJ Brigati (1986) ArticleTitleViral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method Am J Surg Pathol 10 1–8 Occurrence Handle3006523 Occurrence Handle1:STN:280:BimC2Mnkt1U%3D

    PubMed  CAS  Google Scholar 

  9. P Tilston (1997) ArticleTitleAnal human papillomavirus and anal cancer J Clin Pathol 50 625 Occurrence Handle9301544 Occurrence Handle1:STN:280:ByiH2cjgvFc%3D

    PubMed  CAS  Google Scholar 

  10. M Durst L Gissmann H Ikenberg H zur Hausen (1983) ArticleTitleA papillomavirus DNA from a cervical carcinoma and prevalence in cancer biopsy sample from different geographic regions Proc Natl Acad Sci USA 80 3812–15 Occurrence Handle6304740 Occurrence Handle1:STN:280:BiyB387mvVw%3D Occurrence Handle10.1073/pnas.80.12.3812

    Article  PubMed  CAS  Google Scholar 

  11. M Boshart L Gissmann H Ikenberg A Kleinheinz W Scheurlen H zur Hausen (1984) ArticleTitleA new type of human papillomavirus DNA and its presence in genital cancer biopsy and its cell lines derived from cervical cancer EMBO J 3 1151–7 Occurrence Handle6329740 Occurrence Handle1:CAS:528:DyaL2cXktFygtrs%3D

    PubMed  CAS  Google Scholar 

  12. BA Werness AJ Levine PM Howley (1990) ArticleTitleAssociation of human papillomavirus type 16 and 18 E6 proteins p53 Science 248 76–9 Occurrence Handle2157286 Occurrence Handle1:CAS:528:DyaK3cXktF2ltL0%3D

    PubMed  CAS  Google Scholar 

  13. N Dyson PM Howley K Munger E Harlow (1989) ArticleTitleThe human papilloma virus-16 E 7 oncoprotein is able to bind to the retinoblastoma gene product Science 243 934–7 Occurrence Handle2537532 Occurrence Handle1:CAS:528:DyaL1MXhslWjs7k%3D

    PubMed  CAS  Google Scholar 

  14. EA Sisk ES Robertson (2002) ArticleTitleClinical implications of human papillomavirus infection Front Biosci 7 e77–84 Occurrence Handle11861218 Occurrence Handle1:CAS:528:DC%2BD38Xit12qtL4%3D

    PubMed  CAS  Google Scholar 

  15. X Sastre-Garau I Cartier N Jourdan-Da Silva P De Cremoux V Lepage D Charron (2004) ArticleTitleRegression of low-grade cervical intraepithelial neoplasia in patients with HLA-DRB1*13 genotype Obstet Gynecol 104 751–5 Occurrence Handle15458897

    PubMed  Google Scholar 

  16. T Bjørge A Engeland T Luostarinen J Mork RE Gislefoss E Jellum et al. (2002) ArticleTitleHuman papillomavirus infection as a risk factor for anal and perianal skin cancer in a prospective study Br J Cancer 87 61–4 Occurrence Handle12085257 Occurrence Handle10.1038/sj.bjc.6600350

    Article  PubMed  Google Scholar 

  17. C Piketty TM Darragh M Da Costa P Bruneval I Heard MD Kazatchkine et al. (2003) ArticleTitleHigh prevalence of anal human papillomavirus infection and anal cancer precursors among HIV-infected persons in the absence of anal intercourse Ann Intern Med 138 453–9 Occurrence Handle12639077

    PubMed  Google Scholar 

  18. TT Tomson RB Roden TC Wu (2004) ArticleTitleHuman papillomavirus vaccines for the prevention and treatment of cervical cancer Curr Opin Invest Drugs 5 1247–61 Occurrence Handle1:CAS:528:DC%2BD2MXmslyhsg%3D%3D

    CAS  Google Scholar 

  19. R Kagawa T Saito T Miyaoka A Kurokawa N Aritake N Kikkawa (1998) ArticleTitleRelationship between anal and colorectal squamous cell carcinoma and HPV: detection of human papillomavirus by PCR (in Japanese with English abstract) Nihon Daityo-koumonbyo Gakkaizasshi (J Jpn Soc Coloproctol) 51 505–12

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Coloproctology, Rakuwakai Otowa Hospital, 2 Otowa Chinji-cho, Yamashina-ku, Kyoto, 607-8062, Japan

    Ryuzaburo Kagawa

  2. Departments of Surgery, Rakuwakai Otowa Hospital, 2 Otowa Chinji-cho, Yamashina-ku, Kyoto, 607-8062, Japan

    Tetsuya Yamaguchi

  3. Department of Pathology, The Cancer Institute of Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan

    Reiko Furuta

Authors
  1. Ryuzaburo Kagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuya Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Reiko Furuta
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kagawa, R., Yamaguchi, T. & Furuta, R. Histological Features of Human Papilloma Virus 16 and Its Association with the Development and Progression of Anal Squamous Cell Carcinoma. Surg Today 36, 885–891 (2006). https://doi.org/10.1007/s00595-006-3266-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00595-006-3266-x

Key words

Search

Navigation