Abstract
The aim of this study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the pouch mucosa of ulcerative colitis patients in comparison with that in the ileum mucosa of noninflammatory bowel disease patients. Pouch mucosal biopsy specimens were collected from postoperative patients who had undergone surgery for ulcerative colitis. Normal ileum specimens were collected from colon cancer patients. The specimens were assessed by immunofluorescence histochemistry using TLR2, TLR3, TLR4, and TLR5 polyclonal antibodies. The normal ileal mucosa constitutively expressed TLR3 and TLR5, whereas TLR2 and TLR4 were barely detectable. In the mucosa of active pouchitis, TLR2 and TLR4 was strongly upregulated, and TLR4 was upregulated even in a noninflamed pouch. No TLR3 or TLR5 expression was detectable. These data suggest that pouchitis may be associated with distinctive changes in selective TLR expression in the pouch mucosa, and that TLR4 alterations in the innate response system may contribute to the pathogenesis of these disorders in particular.
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DA Macafee JF Abercrombie C Maxwell-Armstrong (2004) ArticleTitlePouchitis Colorectal Dis 6 142–52 Occurrence Handle10.1111/j.1463-1318.2004.00645.x Occurrence Handle1:STN:280:DC%2BD2c3ht1Ohtw%3D%3D Occurrence Handle15109376
MC Rubinstein RL Fisher (1996) ArticleTitlePouchitis: pathogenesis, diagnosis, and management Gastroenterologist 4 129–33 Occurrence Handle1:STN:280:BymA1cvisVI%3D Occurrence Handle8792143
DG Nasmyth PG Godwin MF Dixon NS Williams D Johnston (1989) ArticleTitleIleal ecology after pouch-anal anastomosis or ileostomy. A study of mucosal morphology, fecal bacteriology, fecal volatile fatty acids, and their interrelationship Gastroenterology 96 817–24 Occurrence Handle1:STN:280:BiaC387ktVQ%3D Occurrence Handle2914643
R Medzhitov P Preston-Hurlburt CA Janeway SuffixJr (1997) ArticleTitleA human homologue of the Drosophila Toll protein signals activation of adaptive immunity Nature 24 394–7
O Takeuchi K Hoshino T Kawai H Sanjo H Takada T Ogawa et al. (1999) ArticleTitleDifferential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components Immunity 11 443–51 Occurrence Handle10.1016/S1074-7613(00)80119-3 Occurrence Handle1:CAS:528:DyaK1MXntFCnur4%3D Occurrence Handle10549626
L Alexopoulou AC Holt R Medzhitov RA Flavell (2001) ArticleTitleRecognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3 Nature 413 732–8 Occurrence Handle10.1038/35099560 Occurrence Handle1:CAS:528:DC%2BD3MXnvFegsL0%3D Occurrence Handle11607032
E Cario DK Podolsky (2000) ArticleTitleDifferential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease Infect Immun 68 7010–7 Occurrence Handle10.1128/IAI.68.12.7010-7017.2000 Occurrence Handle1:CAS:528:DC%2BD3MXjslCku7w%3D Occurrence Handle11083826
M Hausmann S Kiessling S Mestermann G Webb T Spottl T Andus et al. (2002) ArticleTitleToll-like receptors 2 and 4 are up-regulated during intestinal inflammation Gastroenterology 122 1987–2000 Occurrence Handle10.1053/gast.2002.33662 Occurrence Handle1:CAS:528:DC%2BD38Xlt12gsLY%3D Occurrence Handle12055604
S Naik EJ Kelly L Meijer S Pettersson IR Sanderson (2001) ArticleTitleAbsence of Toll-like receptor 4 explains endotoxin hyporesponsiveness in human intestinal epithelium J Pediatr Gastroenterol Nutr 32 449–53 Occurrence Handle10.1097/00005176-200104000-00011 Occurrence Handle1:CAS:528:DC%2BD3MXkslGktbs%3D Occurrence Handle11396812
AT Gewirtz TA Navas S Lyons PJ Godowski JL Madara (2001) ArticleTitleCutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression J Immunol 167 1882–5 Occurrence Handle1:CAS:528:DC%2BD3MXlvFeqt7Y%3D Occurrence Handle11489966
G Sebastiani G Leveque L Lariviere L Laroche E Skamene P Gros et al. (2000) ArticleTitleCloning and characterization of the murine toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice Genomics 15 IssueID64 230–40
TG Wolfs WA Buurman A van Schadewijk B de Vries MA Daemen PS Hiemstra et al. (2002) ArticleTitleIn vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation J Immunol 168 1286–93 Occurrence Handle1:CAS:528:DC%2BD38XpsFeltA%3D%3D Occurrence Handle11801667
F Nomura S Akashi Y Sakao S Sato T Kawai M Matsumoto et al. (2000) ArticleTitleCutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with downregulation of surface toll-like receptor 4 expression J Immunol 164 3476–9 Occurrence Handle1:CAS:528:DC%2BD3cXitFOqsrw%3D Occurrence Handle10725699
R Duchmann I Kaiser E Hermann W Mayet K Ewe KH Meyer zum Buschenfelde (1995) ArticleTitleTolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD) Clin Exp Immunol 102 448–55 Occurrence Handle1:STN:280:BymC38fhtlU%3D Occurrence Handle8536356
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Toiyama, Y., Araki, T., Yoshiyama, S. et al. The Expression Patterns of Toll-Like Receptors in the Ileal Pouch Mucosa of Postoperative Ulcerative Colitis Patients. Surg Today 36, 287–290 (2006). https://doi.org/10.1007/s00595-005-3144-y
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DOI: https://doi.org/10.1007/s00595-005-3144-y