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Low levels of ficolin-3 are associated with diabetic peripheral neuropathy

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Abstract

Aims

Ficolin-3, a soluble molecule of the innate immune system, has a primary role in the activation of the lectin pathway in the complement system. Considering that inflammatory mechanisms are involved in complement activation and take part in the pathophysiology of diabetic peripheral neuropathy (DPN), we conducted this study to explore the link between serum ficolin-3 and DPN in diabetic patients.

Methods

A total of 466 diabetic patients were enrolled in this cross-sectional study. DPN was evaluated by neurological symptoms, neurological signs, neurothesiometer and electromyogram. The concentration of serum ficolin-3 was determined by enzyme-linked immunosorbent assay.

Results

The concentration of serum ficolin-3 was lower in DPN patients compared with non-DPN patients (18.73 ± 4.75 vs. 26.69 ± 5.68 ng/mL, P < 0.001). In addition, it was found negatively correlated to the vibration perception threshold (r = −0.158; P = 0.025). The results of multiple regression analysis of DPN indicated that age, diabetes duration, serum ficolin-3 were all independent impact factors for DPN (P < 0.05). Patients were then assigned into quartiles according to the serum ficolin-3 levels, and the prevalence of DPN ascended as the concentration of ficolin-3 descended (Trend analysis, P < 0.001). Compared with ficolin-3 Quartile 1 (referent), the risk of DPN was significantly greater in Quartile 2 (OR 2.76; 95 % CI 1.56–4.88; P < 0.001), Quartile 3 (OR 3.02; 95 % CI 1.69–5.40; P < 0.001) and Quartile 4 (OR 6.84; 95 % CI 3.39–13.80; P < 0.001).

Conclusions

Lower ficolin-3 level is independently associated with DPN, and it may be a potential biomarker for DPN.

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Acknowledgments

This study was supported by the grants from National Science Foundation of China (81270397 for Fang Liu, 81170759 for Lianxi Li).

Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights disclosure

All procedures in this study were in accordance with the ethical standards of the responsible committee on human experimentation of Shanghai Jiao-Tong University Affiliated Sixth People’s Hospital and with the Helsinki Declaration of 1975, as revised in 2008 (5).

Informed consent disclosure

Statement of informed consent was obtained from all patients for being included in the study.

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    Authors and Affiliations

    1. Department of Endocrinology and Metabolism, Shanghai Jiao-Tong University Affiliated Sixth People’s Hospital, Shanghai Clinical Medical Center of Diabetes, Shanghai Key Clinical Center of Metabolic Diseases, Shanghai Institute of Diabetes, Shanghai Key Laboratory of Diabetes, 600 Yishan Road, Shanghai, 200233, China

      Xiaoyan Zhang, Yanyun Hu, Jing Shen, Hui Zeng, Junxi Lu, Lianxi Li, Yuqian Bao, Fang Liu & Weiping Jia

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    1. Xiaoyan Zhang
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    Correspondence to Fang Liu or Weiping Jia.

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    Managed by Massimo Federici.

    X. Zhang and Y. Hu have contributed equally to the work.

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    Zhang, X., Hu, Y., Shen, J. et al. Low levels of ficolin-3 are associated with diabetic peripheral neuropathy. Acta Diabetol 53, 295–302 (2016). https://doi.org/10.1007/s00592-015-0780-6

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