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Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats

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Abstract

There are many medicinal plants in Iranian traditional medicine which are used to treat anemia. One of these plants is Allium eriophyllum Boiss. The aim of this study was to investigate the anti-anemic potential of aqueous extract of A. eriophyllum leaf. After collection of the plant, its extract was obtained using Soxhlet extractor. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of phenylhydrazine in 50 animals. After 1 day, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. eriophyllum at 20, 40, 80, and 160 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were sacrificed, and blood samples were drawn immediately from the animals’ hearts to analyze the biochemical and hematological parameters. The data were analyzed by SPSS-21 software. Different doses of A. eriophyllum significantly (p ≤ 0.05) reduced the raised levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea, creatinine, ferrous, ferritin, and erythropoietin and increased the levels of body weight, white blood cell (WBC), lymphocyte, neutrophils, monocytes, platelet, red blood cell (RBC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) as compared to the untreated group. Seemingly, aqueous extract of A. eriophyllum can be used for the treatment of hemolytic anemia.

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Funding

The study was financially supported by the Kermanshah University of Medical Sciences.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Saman Salmani, Akram Zangeneh, Maryam Almasi & Mohammad Reza Khedri

  2. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

    Mohammad Mahdi Zangeneh & Akram Zangeneh

  3. Department of Medical Laboratory Sciences, School of Paramedicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohsen Zhaleh

  4. Department of Histology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Khodabakhsh Rashidi

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  1. Mohammad Mahdi Zangeneh
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  2. Mohsen Zhaleh
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  3. Saman Salmani
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  4. Akram Zangeneh
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Correspondence to Mohammad Mahdi Zangeneh.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Zangeneh, M.M., Zhaleh, M., Salmani, S. et al. Assessment of the anti-anemic effect of aqueous extract of Allium eriophyllum Boiss leaf in phenylhydrazine-treated Wistar male rats. Comp Clin Pathol 28, 427–434 (2019). https://doi.org/10.1007/s00580-018-2851-x

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  • DOI: https://doi.org/10.1007/s00580-018-2851-x

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