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Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet

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Abstract

The fatty liver disease is one of the most usual metabolic diseases among people around the world. Appropriate strategies for treating this disease are prioritized by each country. The purpose of the new research was to survey the therapeutical property of aqueous extract of Tragopogon graminifolius on the high-fat diet-induced fatty liver disease in Wistar male rats. In this study, 60 rats were used. A total of ten rats were chosen as the negative control, and the rest of them were treated with a high-fat diet for 4 months. Then, the animals were randomly divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the aqueous extract of T. graminifolius at 20, 40, 80, and 160 mg/kg concentrations. After 2 months, the rats were sacrificed, and the blood and liver samples of them were collected for biochemical and histopathological parameter analysis. All doses of T. graminifolius could significantly (p ≤ 0.05) decrease the raised levels of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, and glucose and increased HDL, total protein, albumin, SOD, and CAT as compared to the untreated group. Also, aqueous extract of T. graminifolius decreased the degree of hepatic steatosis as compared to the untreated group. In conclusion, the obtained results demonstrated the hepatoprotective effect of T. graminifolius aqueous extract.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Saman Salmani, Akram Zangeneh, Reza Khedri & Mohammad Saeid Zarei

  2. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

    Mohammad Mahdi Zangeneh & Akram Zangeneh

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  1. Mohammad Mahdi Zangeneh
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  2. Saman Salmani
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  3. Akram Zangeneh
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  4. Reza Khedri
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  5. Mohammad Saeid Zarei
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Correspondence to Mohammad Mahdi Zangeneh.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Zangeneh, M.M., Salmani, S., Zangeneh, A. et al. Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet. Comp Clin Pathol 28, 1197–1203 (2019). https://doi.org/10.1007/s00580-018-2828-9

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  • DOI: https://doi.org/10.1007/s00580-018-2828-9

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