Abstract
The aim of this study was to determine the effect of pycnogenol® (PYC) on erythrocyte stability of donkeys subjected to packing during the late hot-dry season. Ten donkeys, divided into two groups, served as experimental subjects. P-PYC group (n = 5) was subjected to packing only, while P+PYC group (n = 5) was administered with PYC (10 mg/kg) and subjected to packing. Erythrocytes obtained from each animal in the experimental groups were added into saline solution of different concentrations (0.9, 0.6, 0.4. 0.2, and 0.0 g/100 ml). Erythrocyte osmotic fragility index (EOFI) was evaluated spectrophotometrically. The concentration of isoprostane was also determined. The mean EOFI was significantly (P < 0.05) higher in P-PYC group 3 and 7 days after packing at 0.6 g/100 ml, with values of 32.4 ± 3.2 and 53.4 ± 4.3%, respectively. However, there was no significant (P > 0.05) difference in the MCF recorded in both groups, before, after, 3, and 7 days after packing. Percentage erythrocyte stability was significantly higher (P < 0.05) in P+PYC group, especially during, and 7 days after packing with values of 24.3 ± 9.2 and − 23.3 ± 22.8%, respectively when compared with the values of 8.6 ± 3.3 and − 79.7 ± 9.3% obtained in P-PYC group, respectively. Mean isoprostane concentration was significantly (P < 0.05) higher in P-PYC group when compared with the value in P+PYC group, 7 days after packing. In conclusion, environmental and packing stresses decreased stability of the erythrocytes in donkeys; however, PYC may have played an ameliorative role.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig3_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig4_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig5_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig6_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00580-018-2826-y/MediaObjects/580_2018_2826_Fig7_HTML.png)
Similar content being viewed by others
References
Bennett-Wimbush K, Amstutz PM, Willoughby D (2014) Characteristics and welfare of horses used for transportation in northeast Ohio. Prof Anim Sci 30:99–104
Braunwald E (2008) Biomarkers in heart failure. N Engl J Med 358:2148–2159
Cerbo DA, Morales-medina JC, Palmieri B, Pezzuto F, Cocco R, Flores G, Lannilti T (2017) Functional foods in pet nutrition: focus on dogs and cats. Res Vet Sci 112:161–166
Chikezie PC (2007) Osmotic fragility index of HbAA red blood cells in thepresence of aqueous extracts of three medicinal plants (Aframomum melegueta, Garcina kola and Cymbopogon citracus). Global J Pure Appl Sci1 3:496–499
Chikezie PC, Uwakwe AA, Monago CC (2009) Comparative osmotic fragility of three erythrocyte genotypes (HbAA, HbAS and HbSS) of male participants administered with five anti-malaria. Afr J Biochem Res 4:57–64
Czerska M, Zielinski M, Gromadzinska J (2016) Isoprostanes - a novel major group of oxidative stress markers. Int J Occup Med Environ Health 29:179–190
Dzenda T, Ayo JO, Lakpini CAM, Adelaiye AB (2011) Seasonal and sex variations in live weights of captive African Giant rats (Cricetomys gambianus, Waterhouse) in the Northern Guinea Savannah zone of Nigeria. Int J Zool Res 7:49–58
Grimm T, Skrabala R, Chovanova Z, Muchova J, Sumegova K, Liptakova A, Durackova Z, Hogger P (2006) Single and multiple dose pharmacokinetics of maritime pine bark extract (pycnogenol) after oral administration to healthy volunteers. BMC Clin Pharmacol 6:4–15
Gunel C, Demirci B, Eryilmaz A, Yilmaz M, Meteoglu I, Omurlu KI, Basal Y (2016) Inhibitory effect of pycnogenol on airway inflammation in ovalbumin-induced allergic rhinitis. Balkan Med J 33:620–626
Igono MO, Molokwu ECI, Aliu YO (1982) Body temperature responses in Savanna Brown goats to the harmattan and hot-dry seasons. Int J Biometeorol 36:77–87
Krogmeier DE, Mao IL, Bergen WG (1993) Genetic and non-genetic effects of erythrocyte osmotic fragility in lactating Holstein cows and it is associated with yielding traits. J Dairy Sci 76:1994–2000
Lee M, Moon K, Bae D, Park M, Jang A (2013) The effects of pycnogenol on antioxidant enzymes in a mouse model of ozone exposure. Korean J Intern Med 28:216–223
Legha RA, Kumar V, Pai Y, Dedar RK, Bala PA, Ravi SK, Talluri TR, Tripathi BN (2018) Physiological, haematological and biochemical indices in non-descript Indian donkeys working with different pack loads in brick kiln simulated conditions. Int J Liv Res 8:59–66
Margonis K, Fatouros IG, Jamurtas AZ, Nikolaidis MG, Douroudos I, Chatzinikolaou A, Mitrakou A, Mastorakos G, Papassotiriov I, Taxildaris K, Kouretas D (2007) Oxidative stress biomarkers responses to physical overtraining; implications for diagnosis. Free Radic Biol Med 43:901–910
Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84:407–412
Milne GL, Yin H, Hardy KD, Davies SS, Roberts LJ (2011) Isoprostane generation and function. Chem Rev 111:5973–5996
Olaifa FH, Ayo JO, Aluwong T, Rekwot PI, Zakari FO (2018) Ergonomic study of donkeys administered with pycnogenol® and subjected to packing during the hot-dry season in northern Nigeria. Trop Anim Health Prod. https://doi.org/10.1007/s11250-018-1702-8
Oyewale J, Dzenda T, Yaqub L, Akanbi D, Ayo J, Owoyele O, Minka N, Dare T (2011) Alterations in the osmotic fragility of camel and donkey erythrocytes caused by temperature, pH and blood storage. Vet Arhiv 81:459–470
Roberts LJ II, Morrow JD (2000) Measurement of F (2)- isoprostane as an index of oxidative stress in vivo. Free Radic Biol Med 28:505–513
Rohdewald PA (2002) A review of the French maritime pine bark extract (Pycnogenol®), a herbal medication with a diverse clinical pharmacology. Int J Clin Pharmacol Ther 40:158–168
Signolet I, Chenouard R, Oca F, Barth M, Reynier P, Denis M, Simard G (2016) Recurrent isolated haemolytic anaemia: think about glutathione synthetase deficiency. Paediatrics 138:e20154324
Snedecor GW, Cochran WG (1994) Statistical methods. Oxford and IBH Publishing Corporation, Calcutta, p 503
Yang Y, Ahn T, Lee J, Moon C, Kim S, Jun W, Park S, Kim H, Kim J (2008) Protective effects of pycnogenol on carbon-tetrachloride-induced hepatotoxicity in Sprague-Dawley rats. Food Chem Toxicol 46:380–387
Yoshida Y, Umeno A, Shichiri M (2013) Lipid peroxidation biomarkers for evaluating oxidative stress and assessing antioxidant capacity in vivo. J Clin Biochem Nutr 52:9–16
Funding
This study was partially funded by the University of Ilorin, Ilorin, Nigeria.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
The animal experiments within this study were approved by the Institutional Animal Care and Use Committee of Ahmadu Bello University, Zaria, Nigeria and were performed in accordance with the animal welfare guidelines thereof.
Rights and permissions
About this article
Cite this article
Olaifa, F.H., Olusegun, A.J., Tangang, A. et al. Pycnogenol® supplementation improved the erythrocyte stability of packed donkeys during the late hot-dry season in Zaria, Nigeria. Comp Clin Pathol 28, 281–286 (2019). https://doi.org/10.1007/s00580-018-2826-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-018-2826-y