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Evaluation of hematoprotective and hepatoprotective properties of aqueous extract of Ceterach officinarum DC against streptozotocin-induced hepatic injury in male mice

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Abstract

Ceterach officinarum DC has been used in medicine as an antioxidant, anti-inflammatory, antifungal, and antibacterial agent. The aim of the present study was to investigate the hepatoprotective and hematoprotective effects of C. officinarum DC aqueous extract (CO) against streptozocin (STZ)-induced diabetes in mice. In this study, 60 mice were used. Diabetes was experimentally induced by intraperitoneal injection of STZ in 50 mice. Fasting blood glucose levels were assessed everyday by glucometer strips. Mice with plasma glucose level > 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into six groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 50-mg/kg glibenclamide orally. Groups 4, 5, and 6 were given 50, 100, and 200 mg/kg, respectively, of CO for 20 days orally. At 20th day, the mice were dissected and blood and liver samples collected for hematological, biochemical, and histological parameter analysis. Different doses of CO (especially CO200) could significantly (p ≤ 0.05) decreased the raised levels of ALP, AST, ALT, cholesterol, LDL, WBC, and platelet and increased HDL, SOD, CAT, and RBC parameters as compared to the untreated group. The weight and volume of the hepatic structures were decreased significantly (p ≤ 0.05) in different doses of CO as compared to the untreated group. In conclusion, CO has hepatoprotective and hematoprotective properties, thereby reducing the causation of diabetes in experimental mice.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh & Erfan Bahrami

  2. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Akram Zangeneh, Erfan Bahrami, Reza Tahvilian & Rohallah Moradi

  3. Department of Basic Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Akram Zangeneh, Maryam Almasi & Amir Amiri-Paryan

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  1. Mohammad Mahdi Zangeneh
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  2. Akram Zangeneh
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  3. Erfan Bahrami
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Correspondence to Mohammad Mahdi Zangeneh.

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Zangeneh, M.M., Zangeneh, A., Bahrami, E. et al. Evaluation of hematoprotective and hepatoprotective properties of aqueous extract of Ceterach officinarum DC against streptozotocin-induced hepatic injury in male mice. Comp Clin Pathol 27, 1427–1436 (2018). https://doi.org/10.1007/s00580-018-2754-x

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  • DOI: https://doi.org/10.1007/s00580-018-2754-x

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