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Assessment of the subtilisin genes in Trichophyton rubrum and Microsporum canis from dermatophytosis

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Abstract

The presence of subtilisin genes (SUBs) coding for serine proteases with activities keratinolytic on dermatophyte species gives them the ability to adhere and degrade keratin during infection process. The aim of this study was to determine the presence of SUBs in Trichophyton rubrum and Microsporum canis from dermatophyte infections. In the current study, dermatophytes were isolated from human and animal with dermatophytosis. Isolates were identified by direct microscopic and laboratory examination; then, the isolates were examined for the presence of the SUB gene family by PCR with specific primers. This study showed the most frequency of SUB gene families (11/17). At least one virulence gene was observed for 94% (16/17) of the isolates. Statistical analysis revealed significant associations between SUB presence and isolates, origin of isolates (animal or human infection) and infection type (skin, hair, and nail) (P < 0.05). This is the first report on the presence/absence of SUB genes in isolates that were obtained from human and animal infection in Iran, which seems to raise the risk to be pathogenic.

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Acknowledgements

We thank the Department of Medical Mycology Clinical Laboratory in Tehran and Department of Veterinary Microbiology, Shahid Bahonar University of Kerman, Iran.

Funding

This was a self-funded study.

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Authors and Affiliations

  1. Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Azadeh Karami Robati & Mansour Bayat

  2. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University , Kerman, Iran

    Mohammad Khalili

  3. Department of Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Seyyed Jamal Hashemi Hazaveh

Authors
  1. Azadeh Karami Robati
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  2. Mohammad Khalili
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  3. Seyyed Jamal Hashemi Hazaveh
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  4. Mansour Bayat
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Contributions

Azadeh karami Robati, Mohammad Khalili, Seyed Jamal Hashemi Hazaveh, and Mansour Bayat conceived and designed the research. Azadeh karami Robati and Seyed Jamal Hashemi Hazaveh collected samples and clinical data. Azadeh karami Robati performed the research. Azadeh karami Robati analyzed the data. Azadeh karami Robati and Seyed Jamal Hashemi Hazaveh wrote the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Seyyed Jamal Hashemi Hazaveh.

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The authors declare that they have no conflict of interest.

Ethical issues

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Informed consent was obtained from all individual participants included in the study and the Research Committee of Science and Research Branch, Islamic Azad University, Tehran, Iran, approved this study.

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Karami Robati, A., Khalili, M., Hashemi Hazaveh, S.J. et al. Assessment of the subtilisin genes in Trichophyton rubrum and Microsporum canis from dermatophytosis. Comp Clin Pathol 27, 1343–1347 (2018). https://doi.org/10.1007/s00580-018-2745-y

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  • DOI: https://doi.org/10.1007/s00580-018-2745-y

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