Abstract
Anaplasma is a genus of rickettsiales bacteria which is considered as a member of the tick-borne diseases. Different species of this genus are transmitted by blood-sucking insects and arthropods and blood-contaminated instruments. The present study was carried out to determine the prevalence of Anaplasma ovis and Anaplasma marginale and to perform phylogenetic analysis in goat and sheep in East of Kermanshah provinces, western region of Iran. From April 2015 to May 2016, a total of 360 blood samples of small ruminants were collected. In the first step, DNA extraction and in the following nested PCR and the amplification of MSP4 gene were done and a total of 32.78% (118/360) of small ruminants were positive for Anaplasma species. The obtained nucleotide sequences were analyzed and phylogenetic tree was constructed. The similarity among the nucleotide sequences of A. ovis in this study was 100%, and for A. marginale, it showed 99% identity with the others. Statistical analysis by chi-square test did not show any significant relationship between the presence of Anaplasma species and statistical variables like age, tick infestation, sex, and location (p > 0.05). Therefore, further studies are required in different areas of Iran to ascertain our knowledge and to verify Anaplasma species isolates.
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References
Ahmadi-Hamedani M, Khaki Z, Rahbari S, Kazemi B, Bandehpour M (2009) Molecular identification of anaplasmosis in goats using a new PCR-RFLP method. Iranian J Vet Res 10:367–372
Dikmans G (1950) The transmission of anaplasmosis. Am J Vet Res 11:5–16
Ewing SA (1981) Transmission of Anaplasma marginale by arthropods. In: Proceedings of the 7th National Anaplasmosis Conference. Mississippi State University, MS, USA, pp 395–423
Foil LD (1989) Tabanids as vectors of disease agents. Parasitol Today 5:88–96
Hosseini-Vasoukolaei N, Oshaghi MA, Shayan P, Vatandoost H, Babamahmoudi F, Yaghoobi-Ershadi MR, Telmadarraiy Z, Mohtarami F (2014) Anaplasma infection in ticks, livestock and human in Ghaemshahr, Mazandaran Province, Iran. J Arthropod-Borne Dis 8:204–211
Jalali SM, Khaki Z, Kazemi B, Bandehpour M, Rahbari S, Razi Jalali M, Yasini SP (2013) Molecular detection and identification of Anaplasma species in sheep from Ahvaz, Iran. Iranian J Vet Res 14:50–56
Jore S, Viljugrein H, Hofshagen M, Brun-Hansen H, Kristoffersen AB, Nygård K, Ytrehus B (2011) Multi-source analysis reveals latitudinal and altitudinal shifts in range of Ixodes ricinus at its northern distribution limit. Parasit Vectors 4(1):84
Medlock JM, Hansford KM, Bormane A, Derdakova M, Estrada-Peña A, George JC, Kazimirova M (2013) Driving forces for changes in geographical distribution of Ixodes ricinus ticks in Europe. Parasit Vectors 6(1):1
Murray PR, Rosenthal KS, Pfaller MA (2015) Medical microbiology, 8th edn. Elsevier Health Sciences, Philadelphia
Noaman V, Bastani D (2016) Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran. Vet Res Forum 7(2):163–167
Potgieter FT (1979) Epizootiology and control of anaplasmosis in South Africa. J S Afr Vet Assoc 504:367–372
Razmi GR, Dastjerdi K, Hosseini H, Naghibi A, Barati F, Aslani MR (2006) An epidemiological study on Anaplasma infection in cattle, goat and sheep s in Mashhad suburb, Khorasan province, Iran. Ann N Y Acad Sci 1078:479–481
Richey EJ, Palmer GH (1990) Bovine anaplasmosis. Compend Contin Educ Vet 12:1661–1668
Rikihisa Y (1991) The tribe Ehrlichieae and ehrlichial diseases. Clin Microbiol Rev 4:286–308
Robinson MT, Shaw SE, Morgan ER (2009) Anaplasma phagocytophilum infection in a multi-species deer community in the New Forest, England. Eur J Wildl Res 55:439–442
Skerman KD, Hillard JJ (1966) A handbook for Studies of helminth parasites of ruminants. Iran Unit United Nations Development Programme/Special fund Handbook No.2. Near east Animal Health Institutes
Yousefi A, Rahbari S, Sadeghi-dehkordi Z, Bahonar A (2017a) Molecular detection of Anaplasma marginale and Anaplasma ovis in goat and sheep in west highland pasture of Iran. Asian Pac J Trop Biomed 7(5):455–459
Yousefi A, Rahbari S, Sadeghi-dehkordi Z, Bahonar A (2017b) Molecular evidence of Anaplasma phagocytophilum: an emerging tick-borne pathogen in domesticated small ruminant of Iran; first report. Comp Clin Path 26(3):637–642
Acknowledgements
The authors would like to thank Mrs. Fatemeh Khademi, Mis. Fariba nuori, Mr. Abbas Yousefi, and Mr. Masoud Moradi for their help in the collection of samples.
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This study was funded by the Science and Research Branch of Islamic Azad University, Tehran.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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Yousefi, A. Phylogenetic analysis of Anaplasma marginale and Anaplasma ovis isolated from small ruminant based on MSP4 gene in western regions of Iran. Comp Clin Pathol 27, 1161–1165 (2018). https://doi.org/10.1007/s00580-018-2715-4
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DOI: https://doi.org/10.1007/s00580-018-2715-4