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Heat shock protein 70 of Trypanosoma evansi is phylogenitically closer to salivaria than stercoraria homologs

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Abstract

Trypanosomioisis is an economically significant disease affecting a wide range of animals in tropics and subtropics. Heat shock proteins (HSPs) play an important role in trypanosomes by altering parasite’s physiology and adaptability as its life cycle alternates between the poikilothermic invertebrate vector and the homoeothermic vertebrate hosts. The aim of the present study was find out the phylogenetic relationship between T. evansi and other trypanosomes using HSP 70 gene. HSP 70 of Trypanosoma evansi was cloned, characterized, and phylogenitically analyzed with HSP 70 sequences of other trypanosomes. Results revealed that HSP 70 is a highly conserved molecule in trypanosomes. It showed 100% identity with other sequences of T. evansi and T. equiperdum. Alongside, it showed much higher identities with sequences of trypanosomes of anterior station group than those of posterior station group. Hence, it was concluded that HSP 70 of T. evansi is phylogenitically closer to salivaria than stercoraria homologs. The finding is important from evolutionary point of view.

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Acknowledgements

The authors are thankful to Dean and Vice Chancellor, DUVASU, for the facilities provided and for the grant of Institutional project (185/DR/2015) for carrying out the research work. The authors also acknowledge the ICAR for giving various projects to the University for procurement of molecular instruments to carry out research. The authors also acknowledge the help of Dr. Harkirat Singh, GADVASU, and Dr. M. Shanker, IVRI, during sequence analysis.

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Authors and Affiliations

  1. College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Vikrant Sudan, Amit Kumar Jaiswal & Daya Shanker

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  1. Vikrant Sudan
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  2. Amit Kumar Jaiswal
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  3. Daya Shanker
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Correspondence to Vikrant Sudan.

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The blood was collected from the infected animal in compliance with the Standard Ethical Standards. The blood was taken from clinically infected animal whose blood was sent for routine laboratory diagnosis.

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The authors declare that they have no conflict of interest.

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Sudan, V., Jaiswal, A.K. & Shanker, D. Heat shock protein 70 of Trypanosoma evansi is phylogenitically closer to salivaria than stercoraria homologs. Comp Clin Pathol 27, 245–248 (2018). https://doi.org/10.1007/s00580-017-2570-8

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