Abstract
Proper diagnosis of toxoplasmosis is very important for proper treatment in manifested cases and to prevent congenital toxoplasmosis in case of pregnancy. Screening of 180 pregnant females by automated immune assay for Toxoplasma IgG and IgM Abs was done first then positive cases and some of negative cases were evaluated for the Toxoplasma IgG avidity test versus Toxoplasma IgM and IgA Abs using ELISA technique for the detection of active toxoplasmosis during gestation using IHA rising titer as reference test. Assessment of Toxoplasma IgM by ELISA compared with rising titer of IHA as a reference test revealed that the sensitivity, specificity, PPV, and NPP of IgM by ELISA were 66.67, 88.73, 42.86, and 95.45%, respectively. Assessment of Toxoplasma IgA Abs by ELISA compared with rising titer IHA as a reference test showed that the sensitivity, specificity, PPV, and NPP of IgA Abs by ELISA were 44.4, 92.96, 44.4, and 92.96%, respectively. Additionally, by assessment of Toxoplasma IgG avidity compared with rising titer of IHA as a reference test, the sensitivity, specificity, PPV, and NPP of Toxoplasma IgG avidity were 100, 98.59, 90%, and 100%, respectively. Detection of specific Toxoplasma IgM antibodies by ELISA is not always sufficient in the diagnosis of early and late Toxoplasma gondii infection during pregnancy, because Toxoplasma-specific IgM antibodies may persist as long as 18 months after acute acquired infection. Thus, the specific IgG avidity test should be used as more or less low cost tool to detect acute toxoplasmosis.
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References
Abu-Madi MA, Al-Molawi N, Behnke JM (2008) Sero-prevalence and epidemiological correlates of Toxoplasma gondii infections among patients referred for hospital-based serological testing in Doha. Qatar Parasit Vectors 1(1):39–43
Bahar, I. H., Karaman, M., Kirdar, S., Yilmaz, O., Celıloğlu, M and Mutlu, D. (2005): IgA antibodies The importance and validity of anti-Toxoplasma gondii IgG, IgM and IgG avidity tests in the diagnosis of Toxoplasmosis infection during pregnancy Turkey. 29(2): 76–79.
Candolfi E, Pastor R, Huber R, Filisetti D, Villard O (2007) IgG avidity assay firms up the diagnosis of acute toxoplasmosis on the first serum sample in immunocompetent pregnant women. Korean J Parasitol 50(2):99–102
ElDeeb HK, Salah-Eldin H, Khodeer S, Allah AA (2012) Prevalence of Toxoplasma gondii infection in antenatal population in Menoufia governorate. Egypt Acta Trop 124(3):185–191
Elsheikha HM (2008) Congenital toxoplasmosis: priorities for further health promotion action. Public Health 122:335–353
Flori P, Bellete B, Crampe C, Maudry A, Patural H, Chauleur C, Hafid J, Raberin H, Sung R (2008) A technique for dating toxoplasmosis in pregnancy and comparison with the VIDAS anti-toxoplasma IgG avidity test. Clin Microbiol Infect 14(3):242–249
Flori P, Chene G, Varlet MN, Sung RT (2009) Toxoplasma gondii serology in pregnant woman: characteristics and pitfalls. Ann Biol Clin (Paris) 67(2):125–133
Gutierrez, J., Rodriguez, M., Piedrola, G and Maroto, M.C. (2000) Detectionof IgA and low-avidity IgG antibodies for the diagnosis of recent active toxoplasmosis. 3(6).
Hashoosh, D.A and Majeed, I.A. (2014): Comparisonoftwoassaysinthediagnosisoftoxoplasmosis: immunologicalandmolecular. 20(1).
Hussein AH, Ali AE, Saleh MH, Nagaty IM, Rezk AY (2001) Seroprevalence of Toxoplasma infection in Qualyobia governorate, Egypt. J Egypt Soc Parasitol 31:355–363
Kamal AM, Ahmed AK, Abdellatif MZM, Tawfik M, Hassan EE (2015) Seropositivity of toxoplasmosis in pregnant women by ELISA at Minia University Hospital. Egypt Korean J Parasitol 53(5):605–610
Kaul, R., Chen, P and Binder, S.R. (2004):Detection of ImmunoglobulinM antibodies specific for Toxoplasma gondii with increased selectivity for recently acquired infections.; 42 (12):5705–5709.
Lappalainen M, Hedman K (2004) Serodiagnosis of toxoplasmosis. The impact of measurement of IgG avidity. Ann Ist Super Sanita 40(1):81–88
Montoya JG, Liesenfeld PO (2004) Toxoplasmosis Lancet 363(9425):1965–1976
Montoya JG, Liesenfeld O, Kinney S, Press C, Remington JS (2002) VIDAS test for avidity of Toxoplasma-specific immunoglobulin G for confirmatory testing of pregnant women. J Clin Microbiol 40:2504–2508
Roldan, M. M., Heimesaat, M. M and Liesenfeld, L. (2014): Toxoplasmosis Manson tropical disease 23rd edition chapter 48 Elseivirsaunders.
Suzuki AL, Rocha RNJ, Rossi CL (2001) Evaluation of serological markers for the immunodiagnosis of acute acquired toxoplasmosis Sa-o Paulo, Brazil J. Med Microbiol 50:62–70
Tammam AE, Haridy MA, Abdellah AH, Ahmed SR, Fayed HM, Sammani MA (2013) Seroepidemiology of Toxoplasma gondii infection in women with first trimester spontaneous miscarriage in Qena governorate, Egypt. J Clin Diagn Res 7:2870–2873
Werre SR, Jacobson RH, Bowman DD, Dubey JP, Mohammed HO (2002) Evaluation of kinetics and single-read enzyme-linked immunoassays for detection of Toxoplasma gondii antibodies in sheep. J Vet Diagn Investig 14:225–230
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Hamed, A.S.M.R., Omar, S.H., Basyoni, M.M.A. et al. Comparative and analytical study on active toxoplasmosis to assess the IgG avidity in correlation to serological profile in a cohort of Egyptian patients. Comp Clin Pathol 26, 1157–1163 (2017). https://doi.org/10.1007/s00580-017-2501-8
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DOI: https://doi.org/10.1007/s00580-017-2501-8