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The histomorphometric and histopathologic effect of methionine on the epidermis and dermis layers of skin in rat

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Abstract

The skin is the largest organ of the body and is composed of epidermis (the outermost layer of cells consisting mainly of keratinized, stratified, squamous epithelium) and dermis (a layer of connective tissue composed of cells and fibrils such as collagen bundles). The aim of this study was to assess the effect of excess methionine on the histomorphology and histomorphometry of collagen bundles in these layers. Eighty, 9–10-week old, male rats were randomly allocated into eight equal groups. Each group received one of the following treatments: L-methionine (50, 100 or 200 mg/kg B.W.), vitamin C (50 mg/kg B.W), combination of L-methionine (all 3 concentrations) and vitamin C (Met + Vit C), or normal saline as control (CT) for 20 consecutive days. After terminating the animals on day 21, skin samples were taken, and the epidermis, papillary, and reticular layers were evaluated histologically. The data obtained revealed that methionine does not increase collagen bundle thickness unless combined with vitamin C. We suggest that lack of crosslinking occurs when using methionine alone. The greatest increase in both epidermis and dermis occurred in the Met 100 + Vit C group. There was no significant difference between Vit C and control groups. Histopathology did not show any congestion or inflammatory cell infiltration in any treatment groups compared to the controls.

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Acknowledgments

This research was financially supported by the research council of Shahid Bahonar University of Kerman.

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Authors and Affiliations

  1. Department of Basic Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, P. O. Box: 76169133, Kerman, Iran

    Mohammad Naser Nazem

  2. Department of Basic Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Morvarid Teymouri

  3. Department of Clinical Pathology, School of Veterinary Medicine, Zabol University, Zabol, Iran

    Mehdi Jahantigh

Authors
  1. Mohammad Naser Nazem
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  2. Morvarid Teymouri
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  3. Mehdi Jahantigh
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Correspondence to Mohammad Naser Nazem.

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All procedures involving the experimental use of animals were approved by the Animal Ethics Committee, a branch of the Research Council of the Veterinary School in Shahid Bahonar University, Kerman Province, Iran, and administered by the National Animal Ethics Advisory Committee.

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was funded by grant number 1393/10 for scientific research from the Vice Chancellor of Research, Shahid Bahonar University of Kerman, Iran.

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Nazem, M.N., Teymouri, M. & Jahantigh, M. The histomorphometric and histopathologic effect of methionine on the epidermis and dermis layers of skin in rat. Comp Clin Pathol 25, 699–704 (2016). https://doi.org/10.1007/s00580-016-2250-0

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  • DOI: https://doi.org/10.1007/s00580-016-2250-0

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