Selected biochemical parameters in plasma of blood and semen of Persian sturgeon, Acipenser persicus

Abstract

The investigation of blood and seminal plasma biochemical parameters is essential for fish stock conservation and development of artificial propagation methods via extender improvement. In this research, comparison of blood and seminal plasma compounds and their relationship in Persian sturgeon, Acipenser persicus, were studied. Seminal plasma contained 59.53 ± 2.56 mEq/l sodium (Na⁺), 4.72 ± 0.31 mEq/l potassium (K⁺), 1.45 ± 0.075 mEq/l chloride (Cl⁻), 9.1 ± 1.42 mEq/l calcium (Ca²⁺), 0.7 ± 0.072 mEq/l magnesium (Mg²⁺), 0.11 ± 0.021 g/dl total protein, 6.67 ± 1.04 mg/dl cholesterol, 15.2 ± 0.65 mg/dl triacylglycerol, and 18.22 ± 4.16 mg/dl glucose. Also, blood biochemical values were determined (mean ± SEM) for Na⁺ (123.2 ± 1.31 mEq/l), K⁺ (2.77 ± 0.088 mEq/l), Cl⁻ (97.18 ± 1.23 mEq/l), Ca²⁺ (6.67 ± 0.24 mEq/l), Mg²⁺ (1.29 ± 0.074 mEq/l), glucose (198.49 ± 18.03 mg/dl), triacylglycerol (214.22 ± 13.38 mg/dl), total protein (3.62 ± 0.23 g/dl), and cholesterol (127.11 ± 6.94 mg/dl). The mean blood and seminal osmolality values were 244.62 ± 3.66 and 86.9 ± 4.22 mOsm/kg, respectively. Results of the comparison between biochemical parameters of seminal and blood plasma indicated that the concentrations of all parameters of blood plasma were significantly (P < 0.05) higher than seminal plasma except potassium. The statistical Pearson correlation revealed a significant relationship between blood osmolality with levels of Mg⁺⁺ and Na⁺ of the seminal plasma (P < 0.05), and glucose levels of blood and seminal plasma (P < 0.01).