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Sickling, cytomorphology and blood parameters in brown brocket deer (Mazama gouazoubira Fischer, 1814)

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Abstract

Many species of deer, especially North American and Eurasian deer, show a sickling phenomenon (a morphological change in the red blood cells) in in vitro studies, which was subsequently attributed to the existence of different forms of haemoglobin. The objective of this study was to investigate the sickling phenomenon and its cytomorphological basis and, simultaneously, to determine normal blood parameters in captive brown brocket deer (Mazama gouazoubira). Blood was obtained from 30 healthy individuals, 20 males and 10 females. Blood cell morphology was studied with both light and electron microscopic techniques. Determinations of haematological values were done by manual methods. We found the sickling phenomenon to be present in this species in the form of erythrocytes in crescent or holly leaf shape. The scanning electron microscope showed the polymerization of haemoglobin, and the electrophoresis revealed the existence of dimorphic haemoglobin. The number of erythrocytes was larger than reported previously (21.84 ± 3.41 × 106/μl), due to their extremely small size (~3 μm diameter, 18.25 ± 3.15 fl volume). The leucocytes had a typical mammalian morphology. This is the first record of sickling from a Neotropical deer species.

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Acknowledgments

This research was supported by a grant to P. Black-Decima from the Consejo de Investigación de la Universidad Nacional de Tucumán (Research Council of the National University of Tucuman). We thank Alejandra Hurtado for her help with the statistics. We thank the anonymous referees for their helpful comments on the manuscript.

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Authors and Affiliations

  1. Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina

    Silvia Chamut

  2. Reserva Fitozoologica Instituto Dr. Carlos Pellegrini, San Miguel de Tucumán, Tucumán, Argentina

    José Yapur

  3. Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina

    Patricia Black-Decima

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  1. Silvia Chamut
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  2. José Yapur
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  3. Patricia Black-Decima
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Correspondence to Patricia Black-Decima.

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Chamut, S., Yapur, J. & Black-Decima, P. Sickling, cytomorphology and blood parameters in brown brocket deer (Mazama gouazoubira Fischer, 1814). Comp Clin Pathol 23, 1007–1012 (2014). https://doi.org/10.1007/s00580-013-1735-3

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  • DOI: https://doi.org/10.1007/s00580-013-1735-3

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