Abstract
Autoreactive cytotoxic T cells play a key role in the pathogenesis of aplastic anemia (AA) by myelosuppressive cytokines including interferon gamma, tumor necrosis factor alpha, and transforming growth factor beta. As an important cytokine that modulates the cell cycle, the involvement of transforming growth factor beta-1 (TGF-β1) in AA has been studied. The purpose of our study was to investigate the role of (TGF-β1/2509) gene polymorphisms in patients with acquired AA and to assess whether genotype was associated with higher or lower production and compare it with established control populations. Thirty-one patients with acquired aplastic anemia and 30 age- and sex-matched healthy controls were analyzed consecutively using PCR–restriction fragment length polymorphism (PCR-RFLP). TGF-β1 polymorphism C-509T was identified using PCR-RFLP. Significantly different distributions of the gene were demonstrated between the case and the control. The frequencies of −509CT and −509TT genotypes (74.2 versus 30 % and 16.2 versus 3.3 %, respectively) were significantly higher in cases than in controls (P < 0.001), while the frequency of the −509 CC genotype is higher in the control than in cases (66.7 versus 9.6 %, P < 0.001). Our data showed a genotypic profile associated with high TGF-β production in patients with bone marrow failure and suggest that the genetic regulation of inflammatory and T cell-mediated immunological pathways could be involved in the pathogenesis of bone marrow failure, reinforcing the view that AA are organ-specific autoimmune disorders.
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Bacigalupo A, Figari O, Tong J, Piaggio G, Miceli S, Frassoni F, Caciagli P, Badolati G, Marmont AM (1992) Long-term marrow culture in patients with aplastic anemia compared with marrow transplant recipients and normal controls. Exp Hematol 20:425–430
Bazrafshani MR, Ollier WER, Hajeer AH (2000) A novel PCR-RFLP assay for the detection of the single nucleotide polymorphism at position −1082 in the human IL-10 gene promoter. Eur J Immunogenet 27:119–120
Blobe GC, Schiemann WP, Lodish HF (2000) Role of transforming growth factor beta in human disease. N Engl J Med 342:1350–1358
Boesen CC, Radaev S, Motyka SA, Patamawenu A, Sun PD (2002) The 1.1 A crystal structure of human TGF-beta type II receptor ligand binding domain. Structure 10:913–919
Cozen W, Gill PS, Ingles SA et al (2004) IL-6 levels and genotype are associated with risk of young adult Hodgkin lymphoma. Blood 103:3216–3221
Douglas RS, Capocasale RJ, Lamb RJ, Nowell PC, Moore JS (1997) Chronic lymphocytic leukemia B cells are resistant to the apoptotic effects of transforming growth factor-β. Blood 89:941
Dybedal I, Jacobsen SE (1995) Transforming growth factor beta (TGF-beta), a potent inhibitor of erythropoiesis: neutralizing TGF-beta antibodies show erythropoietin as a potent stimulator of murine burst-forming unit erythroid colony formation in the absence of a burst-promoting activity. Blood 86:949
Eaves CJ, Cashman JD, Kay RJ, Dougherty GJ, Otsuka T, Gaboury LA, Hogge DE, Lansdorp PM, Eaves AC, Humphries RK (1991) Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term humanmarrowcultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer. Blood 78:110–117
Fava RA, Casey T, Wilcox J, Pelton RW, Moses, Nanney LB (1990) Synthesis of transforming growth factor-beta 1 by megakaryocytes and its localization to megakaryocyte and platelet alpha-granules. Blood 76:1946–1955
Fermo E, Bianchi P, Barcellini W, Pedotti P, Boschetti C, Alfinito F, Cortelezzis A, Zanella A (2004) Immunoregulatory cytokine polymorphisms in Italian patients affected by paroxysmal nocturnal haemoglobinuria and aplastic anaemia. Eur J Immunogenet 31:267–269
Fracchiolla NS, Barcellini W, Bianchi P, Motta M, Fermo E, Cortelezzi A (2001) Biological and molecular characterization of PNH-like lymphocytes emerging after Campath-1H therapy. Br J Haematol 112:969
Fujiwara M (1990) Long-term cultures of marrow cells from patients with aplastic anaemia. Nippon Ketsueki Gakkai Zasshi—Acta Haematol Jpn 53:533–540
Gascon P, Scala G (1988) Decreased interleukin-1 production in aplastic anemia. Am J Med 85:668–674
Gibson FM, Gordon-Smith EC (1990) Long-term culture of aplastic anaemia bone marrow. Br J Haematol 75:421–427
Gibson FM, Scopes J, Laurie AS, Gordon-Smith EC (1991) Contribution of the marrow stroma to the pathogenesis of aplastic anaemia. Exp Hematol 18:628 (Abstract)
Gidvani V, Ramkissoon S, Sloand EM, Young NS (2004) Cytokine gene polymorphisms in acquired bone marrow failure. Am J Hematol 82:721–724
Gimble JM, Pietrangeli C, Henley A, Dorheim MA, Silver Namen A, Takeichi M, Goridis C, Kincade PW (1989) Characterization of murine bone marrow and spleen-derived stromal cells: analysis of leukocyte marker and growth factor mRNA transcript levels. Blood 74:303–311
Graham GJ, Wright EG, Hewick R, Wolpe SD, Wilkie NM, Donaldson D, Lorimore S, Pragnell IB (1990) Identification and characterization of an inhibitor of haemopoietic stem cell proliferation. Nature 344:442–444
Grainger DJ, Heathcote K, Chiano M et al (1999) Genetic control of the circulating concentration of transforming growth factor type beta1. Hum Mol Genet 8:93–97
Hatzfeld J, Li ML, Brown EL, Sookdeo H, Levesque JP, O’Toole T, Gurney C, Clark SC, Hatzfeld A (1991) Release of early human hematopoietic progenitors from quiescence by antisense transforming growth factor beta 1 or Rb oligonucleotides. J Exp Med 174:925–929
Hoope WC (1991) The role of transforming growth factor-beta in hematopoiesis: a review. Leuk Res 15:179–184
Hotta T, Kato T, Maeda H, Yamao H, Yamada H, Saito H (1985) Functional changes in marrow stromal cells in aplastic anaemia. Acta Haematol 74:65–69
Juneja HS, Lee S, Gardner FH (1989) Human long-term bone marrow cultures in aplastic anemia. Int J Cell Cloning 7:129–135
Kang HG, Chae MH, Park JM et al (2006) Polymorphisms in TGF-beta1 gene and the risk of lung cancer. Lung Cancer 52:1–7
Keller JR, Jacobsen SE, Dubois CM, Hestdal K, Ruscetti FW (1992) Transforming growth factor-beta: a bidirectional regulator of hematopoietic cell growth. Int J Cell Cloning 10:2–11
Kim BC, Mamura M, Choi KS, Calabretta B, Kim SJ (2001) Transforming growth factor beta1 induces apoptosis through cleavage of BAD in a Smad3-dependent mechanism in FaO hepatoma cells. Mol Cell Biol 22:1369
Lawrence DA, Pircher R, Jullien P (1985) Conversion of a high molecular weight latent beta-TGF from chicken embryo fibrobroblasts into a low molecular weight active beta-TGF under acidic conditions. Biochem Biophys Res Commun 133:1026–1034
Lee YG, Kim I, Kim JH, Bae JY, Kwon JH, Shin DY, Lee JE, Song EY, Kim HK, Yoon SS, Park SS, Lee DS, Han KS, Park MH, Hong YC, Park S, Kim BK (2011) Impact of cytokine gene polymorphisms on risk and treatment outcomes of aplastic anemia. Ann Hematol 90:515–521
Maciejewski JP, Anderson S, Katevas P, Young NS (1994) Phenotypic and functional analysis of bone marrow progenitor cell compartment in bone marrow failure. Br J Haematol 87:227–234
Maciejewski JP, Selleri C, Sato T, Anderson S, Young NS (1996) A severe and consistent deficit in marrow and circulating primitive hematopoietic cells (long-term culture-initiating cells) in acquired aplastic anemia. Blood 88:1983–1991
Marsh JC, Chang J, Testa NG, Hows JM, Dexter TM (1990) The hematopoietic defect in aplastic anemia assessed by long-term marrow culture. Blood 76:1748–1757
Marsh JC, Chang J, Testa NG, Hows JM, Dexter TM (1991) In vitro assessment of marrow ‘stem cell’ and stromal cell function in aplastic anaemia. Br J Haematol 78:258–267
Metcalf D (1985) The granulocyte–macrophage colony-stimulating factors. Science 229:16–22
Papadaki HA, Giouremou K, Eliopoulos GD (1999) Low frequency of myeloid progenitor cells in chronic idiopathic neutropenia of adults may be related to increased production of TGF-beta1 by bone marrow stromal cells. Eur J Hematol 63:154
Philpott NJ, Scopes J, Marsh JC, Gordon-Smith EC, Gibson FM (1995) Increased apoptosis in aplastic anemia bone marrow progenitor cells: possible pathophysiologic signifcance. Exp Hematol 23:1642–1648
Polyak K (1996) Negative regulation of cell growth by TGF beta. Biochim Biophys Acta 1242:185–199
Rizzo S, Killick SB, Patel S, Ball SE, Wadhwa M, Dilger P, Gorden-Smith EC (1999) Reduced TGF-b1 in patients with aplastic anaemia in vivo and in vitro. Br J Haematol 107:797–803
Schultz JC, Shahidi NT (1994) Detection of tumor necrosis factor-α in bone marrow plasma and peripheral blood plasma from patients with aplastic anemia. Am J Hematol 45:32–38
Scopes J, Bagnara M, Gordon-Smith EC, Ball SE, Gibson FM (1994) Haemopoietic progenitor cells are reduced in aplastic anaemia. Br J Haematol 86:427–430
Silverman ES, Palmer LJ, Subramaniam V et al (2004) Transforming growth factor-b1 promoter polymorphism C-509T is associated with asthma. Am J Respir Crit Care Med 169:214–219
Taketazu F, Miyagawa K, Ichijo H, Oshimi K, Mizoguchi H, Hirai H, Miyazono K, Takaku F (1992) Decreased level of transforming growth factor-beta in blood lymphocytes of patients with aplastic anemia. Growth Factors 6:85–90
Teramura M, Mizoguchi H (1996) Special education: aplastic anemia. Oncologist 1(3):187–189
Traycoff CM, Orazi A, Ladd AC, Rice S, McMahel J, Srour EF (1998) Proliferation-induced decline of primitive hematopoietic progenitor cell activity is coupled with an increase in apoptosis of ex vivo expanded CD34+ cells. Exp Hematol 26:53–62
Tseng LH, Chen PJ, Lin MT et al (2002) Simultaneous genotyping of single nucleotide polymorphisms in the IL-6, IL-10 TNF-a and TNFb genes. Tissue Antigens 59:280–286
Van Ranst PC, Snoeck HW, Lardon F, Lenjou M, Nijs G, Weekx SF, Rodrigus I, Berneman ZN, Van Bockstaele DR (1996) TGF-beta and MIP-1 alpha exert their main inhibitory activity on very primitive CD34+CD38− cells but show opposite effects on more mature CD34+CD38+ human hematopoietic progenitors. Exp Hematol 24:1509–1515
Zoumbos NC, Gascon P, Djeu JY et al (1985) Interferon is a mediator of hematopoietic suppression in aplastic anemia in vitro and possibly in vivo. Proc Natl Acad Sci U S A 82:188–192
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Afify, R.A.A., Salama, N. Correlation of transforming growth factor beta-1 gene polymorphisms C-509T and aplastic anemia. Comp Clin Pathol 22, 755–760 (2013). https://doi.org/10.1007/s00580-012-1478-6
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DOI: https://doi.org/10.1007/s00580-012-1478-6