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Alterations of serum tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and sialic acids in malignant ovine theileriosis

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Abstract

The study was designed to evaluate the effect of the severity of Theileria lestoquardi (hirci) infection on tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and sialic acid concentration in blood sera in a naturally infected sheep. Infected animals (diseased group) comprised 50 Iranian fat-tailed sheep, about 1–2 years old, naturally infected with Theileria lestoquardi (hirci), were divided into three subgroups according to parasitemia rates (<2%, 2–4%, and >4%). As a control group, ten noninfected sheep were also selected. Hematological parameters and levels of TNF-α, IFN-γ, and sialic acid (TSA, LBSA, and PBSA) concentrations were measured in both groups. The results showed significant increases in the level of TNF-α, IFN-γ, and sialic acid concentrations in infected sheep compared with noninfected ones (P < 0.05). The increased level of TNF-α and IFN-γ in infected sheep may indicate the role of these cytokines to inhibit the growth and development of the parasite in infected host cells. Also, it is speculated that preventing the establishment of parasites by inhibiting the differentiation of trophozoites to schizonts is another aspect of the enhanced activity of these substances. In addition, increased levels of sialic acid concentration during parasitemia presumably stimulate the host immune response and influence the parasite–host cell adhesion. Clearly, further molecular and biochemical investigations are needed to elucidate the exact mechanisms involved in immunopathogenesis of malignant ovine theileriosis.

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Abbreviations

IFN-γ:

interferon-γ

LBSA:

lipid bound sialic acid

PBSA:

protein bound sialic acid

TSA:

total sialic acid

TNF-α:

tumor necrosis factor-α

References

  • Aggarwal BB, Eessalu TE, Hass PE (1985) Characterization of receptors for human tumor necrosis factor and their regulation by gamma-interferon. Nature 318:665–667

    Article  CAS  PubMed  Google Scholar 

  • Ahmed JS (2002) The role of cytokines in immunity and immunopathogenesis of pirolasmoses. Parasitol Res 88:48–50

    Article  Google Scholar 

  • Ahmed JS, Mehlhorn H (1999) The cellular basis of the immunity to and immunopathogenesis of tropical theileriosis. Parasitol Res 85:539–549

    Article  CAS  PubMed  Google Scholar 

  • Brown CGD, Ilhan T, Kirvar E, Thomas M, Wilkie G, Leemans I, Hooshmand-Rad P (1998) Theileria lestoquardi and T. annulata in cattle, sheep, and goats, in vitro and in vivo studies. Ann N Y Acad Sci 849:44–51

    Article  CAS  PubMed  Google Scholar 

  • Campbell JDM, Brown DJ, Nichani AK, Howie SEM, Spooner RL, Glass EJ (1997) A non-protective T helper 1 response against the intra-macrophage protozoan Theileria annulata. Clin Exp Immunol 108:463–470

    Article  CAS  PubMed  Google Scholar 

  • Crook M (1993) The determination of plasma or serum sialic acid. Clin Biochem 26:31–38

    Article  CAS  PubMed  Google Scholar 

  • Dall'olio F (2000) The sialyl-2, 6-lactosaminyl-structure: biosynthesis and functional role. Glycoconj J 17:669–676

    Article  PubMed  Google Scholar 

  • DeMartini JS, Baldwin CL (1991) Effects of gamma interferon, tumor necrosis factor alpha, and interleukin-2 on infection and proliferation of Theileria parva-infected bovine lymphoblasts and production of interferon by parasitized cells. Infect Immun 59:4540–4546

    CAS  PubMed  Google Scholar 

  • Emery DL (1981) Adoptive transfer of immunity to infection with Theileria parva (East Coast Fever) between cattle twins. Res Vet Sci 30:365–367

    Google Scholar 

  • Guzel M, Askar TA, Kaya G, Atakisi E, Avci GE (2008) Serum sialic acids, total antioxidant capacity, and adenosine deaminase activity in cattle with theileriosis and anaplasmosis. Bull Vet Inst Pulawy 52:227–230

    Google Scholar 

  • Haq M, Haq S, Tutt P, Crook M (1993) Serum total sialic acid and lipid-associated sialic acid in normal individuals and patients with myocardial infarction and their relationship to acute phase proteins. Ann Clin Biochem 30:383–386

    PubMed  Google Scholar 

  • Jain NC (1986) Schalms veterinary hematology, 4th edn. Lea & Febiger Publication, Philadelphia, pp 20–80

    Google Scholar 

  • Jain NC (1993) Essentials of veterinary hematology, 1st edn. Lea & Febiger Publication, Philadelphia, pp 389–396

    Google Scholar 

  • Karagenc TI, Kiral FK, Seyrek K, Bildik A, Eren H (2005) Detection of serum total sialic acid in cattle with natural tropical theileriosis. Revue Méd Vét 156:578–582

    CAS  Google Scholar 

  • Katopodis N, Hirshaut Y, Geller NL, Stock CC (1982) Lipid-associated sialic acid test for the detection of human cancer. Cancer Res 42:5270–5275

    CAS  PubMed  Google Scholar 

  • Landolfo S, Gariglio M, Gribaudo G, Jemma C, Giovarelli M, Cavallo G (1988) Interferon-gamma is not an antiviral, but a growth promoting factor for T lymphocytes. Eur J Immunol 18:503–509

    Article  CAS  PubMed  Google Scholar 

  • Lee SH, Aggarwal BB, Rinderknecht E, Assisi F, Chiu H (1984) The synergistic anti-proliferative effect of gamma-interferon and human lymphotoxin. J Immunol 133:1083–1086

    CAS  PubMed  Google Scholar 

  • Malykhy YN, Schauer R, Shaw L (2001) N-glycolylneuraminic acid in human tumors. Biochimie 83:623–634

    Article  Google Scholar 

  • Mehta HK, Sisodia RS, Misraula RS (1988) Clinical and haematological observations in experimentally induced cases of bovine theileriosis. Indian J Anim Sci 58:584–587

    Google Scholar 

  • Paliard X, Malefijt R, Yssel H, Blanchard D, Chretien I, Abrams J, DeVries J, Spits J (1988) Simultaneous production of IL-2, IL-4 and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol 141:849–855

    CAS  PubMed  Google Scholar 

  • Preston PM, Brown CGD, Richardson W (1992) Cytokines inhibit the development of trophozoite-infected cells of Theileria annulata and Theileria parva but enhance the proliferation of macroschizont-infected cell lines. Parasite Immunol 14:125–141

    Article  CAS  PubMed  Google Scholar 

  • Rayula V, Hafeez MD (1995) Haematological values in cattle infected with Theileria annulata. Indian J Anim Sci 56:1202–1203

    Google Scholar 

  • Razi N, Varki A (1998) Masking and unmasking of the sialic acid-binding lectin activity of CD22 (Siglec-2) on B lymphocytes. Proceeding of National Academy Science USA 95:7469–7474

    Article  CAS  Google Scholar 

  • Ruggiero V, Latham K, Baglioni C (1987) Cytostatic and cytotoxic activity of tumor necrosis factor on human cancer cells. J Immunol 138:2711–2717

    CAS  PubMed  Google Scholar 

  • Schnittger L, Hong Y, Jianxun L, Ludwig W, Shayan P, Rahbari S, Voss-Holtman A, Ahmed JS (2000) Phylogenetic analysis by rRNA comparison of the highly pathogenic sheep-infecting parasites Theileria lestoquardi and a Theileria species identified in China. Ann N Y Acad Sci 916:271–275

    Article  CAS  PubMed  Google Scholar 

  • Shiono H, Yagi Y, Chikayama Y, Miyazaki S, Nakamura I (2003) Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti. Parasitol Res 89:228–234

    PubMed  Google Scholar 

  • Siegel JP (1988) Effects of interferon-γ on the activation of human T-lymphocytes. Cell Immunol 111:461–472

    Article  CAS  PubMed  Google Scholar 

  • Singh A, Singh J, Grewal AS, Brar RS (2001) Studies on some blood parameters of crossbred calves with experimental Theileria annulata infections. Vet Res Commun 25:289–300

    Article  CAS  PubMed  Google Scholar 

  • Soulsby EJL (1982) Helminthes, arthropods and protozoa of domesticated animals, 7th edn. Bailliere Tindal, London, pp 728–739

    Google Scholar 

  • Spits H, Paliard X, Engelhard VH, De Vries JE (1990) Cytotoxic activity and lymphokine production of T cell receptor (TcR)-αβ+ and TcR-γδ+ cytotoxic T lymphocyte (CTL) clones recognizing HLA-A2 and HLA-A2 mutans. J Immunol 144:4156–4162

    CAS  PubMed  Google Scholar 

  • Stockham SL, Kjemtrup AM, Conrad PA, Schmidt DA, Scott MA, Robinson TW, Tyler JW, Johnson GC, Carson CA, Cyddugee P (2000) Theileriosis in a Missouri beef herd caused by Theileria buffeli :case report, herd investigation ultrastructure, phylogenetic analysis and experimental transmission. Vet Pathol 37:11–21

    Article  CAS  PubMed  Google Scholar 

  • Sugarman BJ, Aggarwal BB, Hass PE, Figari IS, Palladino MA, Shepard HM (1985) Recombinant human tumor necrosis-α: effects on proliferation of normal and transformed cells in vitro. Science 230:943–945

    Article  CAS  PubMed  Google Scholar 

  • Suzuki Y, Orellana MA, Schreiber RD, Remington JS (1988) Interferon-γ: the major mediator of resistance against Toxoplasma gondii. Science 240:515–518

    Article  Google Scholar 

  • Vergara U, Ferreira A, Schellekens H, Nussenzweig V (1987) Mechanism of escape of erythrocytic forms (EEF) of malaria parasites from the inhibitory effects of Interferon-gamma. J Immunol 138:4447–4449

    CAS  PubMed  Google Scholar 

  • Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chemis 234:1971

    CAS  Google Scholar 

  • Yurtseven S, Uysal H (2009) Decreased serum sialic acid, albumin-globulin ratio and total protein levels in cattle heavily infected with Theileria annulata. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56:141–144

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz, 71345, Iran

    S. M. Razavi, M. Masoudian & E. Rakhshandehroo

  2. Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    S. Nazifi & F. Vosoughi

  3. Department of Clinical Studies, School of Veterinary Medicine, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran

    A. Nowroozi Asl

Authors
  1. S. M. Razavi
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  2. S. Nazifi
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  3. F. Vosoughi
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  4. M. Masoudian
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  5. A. Nowroozi Asl
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  6. E. Rakhshandehroo
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Correspondence to S. M. Razavi.

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Razavi, S.M., Nazifi, S., Vosoughi, F. et al. Alterations of serum tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and sialic acids in malignant ovine theileriosis. Comp Clin Pathol 19, 503–509 (2010). https://doi.org/10.1007/s00580-009-0917-5

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  • DOI: https://doi.org/10.1007/s00580-009-0917-5

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