Abstract
Cervical mucosa cytology and hematological parameters in heifers were determined during different phases of the estrous cycle. Ten healthy Holstein heifers were synchronized by two injections of PGF2α at 14 days interval. Blood and cervical mucosa samples were collected at days of 0, 2, 10 and 19 of estrous cycle. Differential cell counts were performed, and the progesterone level was measured by radioimmunoassay. Progesterone levels in diestrus and the percentages of neutrophils in metestrus were shown to be higher than in other phases of the estrous cycle (P<0.05). In contrast, the percentages of epithelial cells in metestrus were lower than other phases (P<0.05). A difference was also seen (P<0.05) in blood progesterone levels between four estrous cycle phases. However, there was no significant difference in RBCs, hemoglobin, hematocrit, WBCs, neutrophils, lymphocytes, monocytes, and eosinophils between four estrous cycle phases. Hormonal changes in different phases of estrous cycle affect neutrophil presence in cervical mucosa. It is important to consider cervical cytology and hormonal assay together for evaluation of dairy cow reproductive tract function.
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Ahmadi MR, Nazifi S, Khodakaram Tafti A (2004) Cytological studies of uterus and uterine cervical mucosa by swab and aspiration methods in cows. J Fac Vet Med Univ Tehran 59:360–366
Ahmadi MR, Kafi M, Ghodrat M (2005) Crystallization and the number of neutrophils increase in the cervical mucus as parturition approaches in dairy cows. Com Clin Pathol 14(2):72–75
Black WG, Ulberg LC, Kidder HE, Simon J, McNutt SH, Casida LE (1953) Inflammatory responses of the bovine endometrium. Am J Vet Res 15:247–251
Faundez R, Duszewska AM, Klucinski W, Spohr I, Sitarska E, Trenti F (1994) Occurrence of leukocytes and epithelial cells in the lumen of the reproductive tract during the ovarian cycle. Proceeding 18th World Buiatrics Congress: 26th Congress of the Italian Association of Buiatrics, Bologna, Italy
Ford SP (1982) Control of uterine and ovarian blood flow throughout the estrous cycle and pregnancy of ewes, sows and cows. J Anim Sci 55(suppl):32–42
Ford SP, Chenault JR, Echternkamp SE (1979) Uterine blood flow of cows during the oestrous cycle and early pregnancy: effect of the conceptus on the uterine blood supply. J Reprod Fertil 56:53–62
Hafez B, Hafez ESE (2000) Anatomy of female reproduction. In: Hafez B, Hafez ESE (eds) Reproduction in farm animals. Lippincott Willams & Wilkins, pp 13–29
Hoedemaker M, Lund LA, Wagner WC (1992) Influence of arachidonic acid metabolites and steroids on function of bovine polymorphonuclear neutrophils. Am J Vet Res 53:1534–1539
Jain NC (1986) Schalm’s veterinary haematology. Lea and Febiger, Philadelphia, pp 335–345
Jainudeen MR, Hafez ESE (2000) Cattle and buffalo. In: Hafez B, Hafez ESE (eds) Reproduction in farm animals, 7th edn. Lippincott Williams & Wilkins, Maryland, USA, pp 159–171
Johnson SK, Johnson AR, Keefer CL, Silcox RW (1990) Blood constituents during the estrous cycle and early pregnancy in dairy cows. Theriogenology 34:701–707
Kasimanickam R, Duffield TF, Foster RA, Gartley CJ, Leslie KE, Walton JS, Johnson WH (2004) Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology 62:9–23
Lander Chacin MF, Hansen PJ, Drost M (1990) Effects of stage of oestrous cycle and steroid treatment on uterine immunoglobulin content and polymorphonuclear leukocytes in cattle. Theriogenology 34:1169–1184
Niswender GD, Reimers TJ, Diekman MA, Nett TM (1976) Blood flow: a mediator of ovarian function. Biol Reprod 13:381
Noakes DE, Parkinson TJ, England GCW (2001) Arthur’s veterinary obstetrics, 8th edn. Saunders, Philadelphia, PA, pp 189–202
Raab D, Drillich M, Heuwieser W (2002) Diagnosis of subclinical endometritis and its effect on reproductive performance. XXII World Buiatrics Congress. Hanover, Germany
Roman-Ponce H, Caton D, Thatcher WW, Lehrer R (1983) Uterine blood flow in relation to endogenous hormones during estrous cycle and early pregnancy. Am J Physiol 245:843–849
Roth JA, Kaeberle ML, Appell LB, Nachreiner RF (1983) Association of increased oestradiol and progesterone blood values with altered bovine polymorphonuclear leukocyte function. Am J Vet Res 44:247–253
Rowson LEA, Lamming GE, Fry RM (1953) The relationship between ovarian hormones and uterine infection. Vet Rec 65:355
Subandrio AL, Noakes DE (1992) The influence of the stage of the bovine oestrous cycle on the chemotactic stimulus of oyster glycogen to intrauterine neutrophils. Br Vet J 148:163–165
Subandrio AL, Noakes DE (1997) Neutrophil migration into the uterine lumen of the cow: the influence of endogenous and exogenous sex steroid hormones using two intrauterine chemoattractants. Theriogenology 47:825–835
Subandrio AL, Sheldon IM, Noakes DE (2000) Peripheral and intrauterine neutrophil function in the cow: the influence of endogenous and exogenous sex steroid hormones. Theriogenology 53:1591–1608
Wise TH, Caton D, Thatcher WW, Barron DH, Fields MJ (1982) Ovarian function during the estrus of the cow: ovarian blood flow and progesterone release rate. J Anim Sci 55:627–637
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Ahmadi, M.R., Nazifi, S. & Ghaisari, H.R. Comparison of hormonal changes of estrous cycle with cytology of cervical mucosa and hematological parameters in dairy heifers. Comp Clin Pathol 15, 94–97 (2006). https://doi.org/10.1007/s00580-006-0613-7
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DOI: https://doi.org/10.1007/s00580-006-0613-7