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Interactions among Glomus irregulare, arbuscular mycorrhizal spore-associated bacteria, and plant pathogens under in vitro conditions

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Abstract

Arbuscular mycorrhizal (AM) fungi interact with bacteria (AM fungi-associated bacteria, AMB) in the mycorrhizosphere. We previously identified a set of AMB that enhance AM fungal colonization, plant growth, and inhibit pathogens. Here, we used transformed carrot root cultures in a two-compartment plate system for further in vitro studies on interactions taking place among Glomus irregulare (syn.Glomus intraradices), AMB, and plant pathogens. We found that exudates of G. irregulare stimulated growth of all ten AMB isolates tested in multi-well plates. AMB growth stimulation was observed also during co-cultivation of three of these AMB with G. irregulare in the hyphal compartment. In addition, co-cultivation stimulated growth of G. irregulare hyphae and spore production, as well as G. irregulare root colonization. GC/MS analysis in a preliminary screening of metabolites revealed differences in concentrations of several identified but also unidentified compounds in G. irregulare hyphal exudates. Exudates in presence of three different AMB isolates co-cultivated with G. irregulare contained several additional compounds that differed in amount compared with G. irregulare alone. The results indicate that G. irregulare exudates contain carbohydrates, amino acids, and unidentified compounds that could serve as a substrate to stimulate AMB growth. With regard to effects on plant pathogens, growth inhibition of Rhizoctonia solani, Verticillium dahliae, and Pectobacterium carotovorum ssp. carotovorum was evident in the presence of the AMB isolates tested together with the G. irregulare exudates. These in vitro studies suggest that G. irregulare and AMB stimulate growth of each other and that they together seem to provide an additive effect against growth of both fungal and bacterial pathogens.

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Acknowledgments

We thank Krister Lundgren, Umeå Plant Science Centre, Umeå University, for excellent metabolite analysis. This work was financially supported by the Strategic Programme on Interactions between Micro-organisms and Plants (IMOP) at the Swedish University of Agricultural Sciences (SLU), Uppsala, the Carl Tryggers Foundation for Scientific Research, Stockholm, Stiftelsen Lantbruksforskning (SLF), Stockholm, and the Swedish Board of Agriculture (SJV), Norrköping.

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Authors and Affiliations

  1. Department of Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-750 07, Uppsala, Sweden

    Dharam Parkash Bharadwaj & Sadhna Alström

  2. Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7080, SE-750 07, Uppsala, Sweden

    Per-Olof Lundquist

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  1. Dharam Parkash Bharadwaj
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  2. Sadhna Alström
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  3. Per-Olof Lundquist
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Correspondence to Per-Olof Lundquist.

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Bharadwaj, D.P., Alström, S. & Lundquist, PO. Interactions among Glomus irregulare, arbuscular mycorrhizal spore-associated bacteria, and plant pathogens under in vitro conditions. Mycorrhiza 22, 437–447 (2012). https://doi.org/10.1007/s00572-011-0418-7

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