Abstract
The synergistic benefits of the dual inoculation of legumes with nodule bacteria and arbuscular mycorrhizae (AM) are well established, but the effect of an external NH +4 supply on this tripartite relationship is less clear. This effect of NH +4 supply was investigated with regards to the growth and function of the legume host and both symbionts. Nodulated Phaseolus vulgaris seedlings with and without AM, were grown in a sand medium with either 0 N, 1 mM or 3 mM NH +4 . Plants were harvested at 30 days after emergence and measurements were taken for biomass, N2 fixation, photosynthesis, asparagine concentration, construction costs and N nutrition. The addition of NH +4 led to a decline in the percentage AM colonization and nodule dry weights, although AM colonization was affected to a lesser extent. NH +4 supply also resulted in a decrease in the reliance on biological nitrogen fixation (BNF); however, the AM roots maintained higher levels of NH +4 uptake than their non-AM counterparts. Furthermore, the non-AM plants had a higher production of asparagine than the AM plants. The inhibitory effects of NH +4 on nodule function can be reduced by the presence of AM at moderate levels of NH +4 (1 mM), via improving nodule growth or relieving the asparagine-induced inhibition of BNF.
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Mortimer, P.E., Pérez-Fernández, M.A. & Valentine, A.J. Arbuscular mycorrhiza maintains nodule function during external NH +4 supply in Phaseolus vulgaris (L.). Mycorrhiza 22, 237–245 (2012). https://doi.org/10.1007/s00572-011-0396-9
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DOI: https://doi.org/10.1007/s00572-011-0396-9